Cut Site:
GCCN NNN▼NGGC
CGGN▲NNN NCCG
Notes:
- BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Incubation Conditions: Buffer D. 37°C.
Source: Bacillus globigii.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.