DpnI

Cut Site:

G^meA^▼TC
CT_▲^meAG

Usage Note: DpnI requires N⁶-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.

Incubation Conditions: Buffer B. 37°C.

Source: Diplococcus pneumoniae.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
50–75%	100%	75–100%	50–75%	100%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
116	87	0	15	7	22

Protocols

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Buffer B 10X Buffer	R002A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
DpnI	R623A	1 × 200u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Other Resources

How to Use Restriction Enzymes: A Resource Guide

Frequently Used With

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

Wizard® Genomic DNA Purification Kit

Manual, solution-based method for extracting DNA from many sample types.

A1120, A1125, A1620

HDAC-Glo™ I/II Assays and Screening System

Detects HDAC class I and II enzyme activity.

G6420, G6421, G6422