HindIII

Cut Site:

A^▼AGCT   T
T   TCGA_▲A

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Note: Star activity may be observed in the presence of Mn²⁺.

Incubation Conditions: Buffer E. 37°C.

Source: This enzyme is purified from an E. coli strain that carries the HindIII gene from Haemophilus influenzae Rd (ATCC 51907).

Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: