MluI

Cut Site:

A^▼CGCG T
T GCGC_▲A

Usage Note: Ends generated using MluI can be directly ligated to ends generated by BssHII. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Micrococcus luteus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
10–25%	25–50%	50–75%	100%	10–25%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
7	5	2	0	0	0

Protocols

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Buffer D 10X Buffer	R004A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
MluI	R638A	1 × 1,000u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Citations

The conserved active site asparagine in class I ribonucleotide reductase is essential for catalysis.
--
2002 J. Biol. Chem.
See all citations

Other Resources

How to Use Restriction Enzymes: A Resource Guide

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Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

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Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041