Cut Site:
G▼TCGA C
C AGCT▲G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated by SalI can be directly ligated to ends generated by XhoI. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Streptomyces albus G.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.