Cut Site:
CCC▼GGG
GGG▲CCC
Note: XmaI is a neoschizomer of SmaI.
Incubation Conditions: Buffer J. 25°C. (SmaI exhibits 25–50% activity at 37°C.)
Source: Serratia marcescens.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.