Glycogen-Glo™ Assay

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j5052-glycogen-glo-assay-50-ml
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Easy, Sensitive Glycogen Assay Kit

  • Compatible with cell lysates and tissue homogenates
  • In-well sample preparation protocol streamlines workflow
  • Scalable to 384-well plates for HTS applications

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Catalog number selected: J5051

$ 505.00
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Glycogen-Glo™ Assay
5ml
$ 505.00
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Efficient Glycogen Detection from Diverse Samples

The Glycogen-Glo™ Assay is a fast and sensitive method for detecting glycogen from various biological samples, such as cultured cells and tissue homogenates. Based on bioluminescent technology, the Glycogen-Glo™ Assay can detect subtle changes in glycogen synthesis, storage and breakdown. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.

How the Glycogen-Glo™ Assay Works

Diagram illustrating how the Glycogen-Glo™ Assay works.

The Glycogen-Glo™ Assay utilizes a series of enzyme-coupled reactions. First, glycogen is digested into glucose using glucoamylase enzyme. The resulting glucose is measured using glucose dehydrogenase in conjunction with a bioluminescent NADH detection technology. The result is a light signal proportional to the starting glycogen concentration in the sample.

How to Determine Glycogen Concentration

Samples that contain both glycogen and glucose require two reactions to determine the glycogen concentration.

Samples that contain both glycogen and glucose require two reactions to determine the glycogen concentration. One reaction is used to measure total glucose resulting from digested glycogen plus any glucose present in the sample. The second is used to measure any glucose that was present in the sample prior to digestion. The difference in signal between the two reactions represents the contribution from glycogen in the sample. The effective digestion of glycogen into glucose monomers (≥70%) simplifies data interpretation and calculations.

Simple Assay Protocol Saves Time

Diagram illustrating the Glycogen-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in Glycogen

Graph showing the linear relationship between luminescence and glycogen concentration.

 

Sensitivity (S/B >5)

0.6µg/ml

Limit of Detection (S/N >3)

20ng/ml

Linear Range

To 20µg/ml glycogen

Maximum Assay Window (S/B)

>150

Measure Glycogen Content of Cells in Culture

Graph showing the linear luminescent signal for glycogen concentration in three cell lines.
Graph showing the linear relationship between log luminescence and log cell quantity for measuring glycogen concentration in three cell lines.

Cellular glycogen content varies greatly depending on cell type and growth conditions. The high sensitivity and wide linearity of the Glycogen-Glo™ Assay accommodate measurement of glycogen in cells, regardless of whether they have low or high glycogen levels. The data show analysis of intracellular glycogen content of three cell lines using the Glycogen-Glo™ Assay.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

Explore the Cellular Energy Metabolism Portfolio

Protocols

Complete Protocol

Specifications

You are viewing: J5051 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase

 G884A 1 × 55μl 6mg/ml

Reductase Substrate

 G885A 1 × 55μl

Luciferin Detection Solution

 J135A 1 × 5ml

NAD

 J136A 1 × 30μl

Glucose

 J138A 1 × 50μl

Glucose Dehydrogenase

 J140A 1 × 200μl

Glucoamylase Buffer

 J403A 1 × 3ml

Glucoamylase

 J404A 1 × 25μl

Glycogen

 J405A 1 × 50μl

0.3N HCl

 J406A 1 × 15ml

Tris Buffer

 J407A 1 × 15ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: J5052 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase

 G884B 1 × 275μl 6mg/ml

Reductase Substrate

 G885B 1 × 275μl

Luciferin Detection Solution

 J135B 1 × 50ml

NAD

 J136B 1 × 275μl

Glucose

 J138A 1 × 50μl

Glucose Dehydrogenase

 J140B 2 × 1ml

Glucoamylase Buffer

 J403B 1 × 30ml

Glucoamylase

 J404B 1 × 150μl

Glycogen

 J405A 1 × 50μl

0.3N HCl

 J406A 1 × 15ml

Tris Buffer

 J407A 1 × 15ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Articles

Posters

Other Resources

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