The MTase-Glo™ Methyltransferase Assay is a novel, bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in many applications, including high-throughput screening.
Monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases.
Used successfully for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.