PowerSeq® Quant MS System

PS5000_PowerSeq Quant MS_3
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Quantification of MiSeq® Platform Compatible MPS Libraries

  • Enables normalization of MPS libraries based on DNA quantification
  • Uses BRYT Green® dye-based qPCR system for maximum sensitivity and reproducibility
  • Enables accurate and balanced multiplexed Illumina pooled libraries

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Catalog number selected: PS5000

$ 789.00
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PowerSeq® Quant MS System
500 reactions
$ 789.00
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Consistent Quantification for MPS Libraries

The PowerSeq® Quant MS System is a qPCR-based system designed for library quantification within the MPS workflow by directly measuring the number of DNA molecules present in your libraries. Accurate quantification of a library determines the optimal amount of adapter ligated fragments for cluster generation and ensures even representation of each library on the chip.

Balanced Representation of Pooled Libraries

Use of the PowerSeq® Quant MS System ensures that your libraries are evenly represented and precisely quantified on the MiSeq® flowcell. This results in more consistent and reproducible sequencing results.

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Thirty-two libraries were quantified using the PowerSeq® Quant MS System. The resulting values were used to normalize loading of samples into a final sequencing pool. The percent of reads identified for each sample from the total reads generated in the sequencing run demonstrates how evenly each sample was represented on the MiSeq® flowcell in relation to all libraries sequenced.

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

PowerSeq® Quant 2X Master Mix

 DP115A 5 × 1ml

PowerSeq® Quant MS 10X Primer Mix

 DK803A 1 × 1ml

PowerSeq® Quant MS DNA Standard

 DD104A 1 × 200μl 200pM

PowerSeq® Quant MS 1X Dilution Buffer

 DM146A 2 × 150ml

Water, Amplification Grade

 DW099A 2 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Frequently Used With

PowerSeq® 46GY System

Combined globally used autosomal STR loci with 23 Y-STR loci in a massively parallel sequencing (MPS) application.

PS4600

PowerSeq® CRM Nested System, Custom

Produce MPS libraries ready for mtDNA analysis.

AX5810

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