PowerPlex® Fusion 6C System

Highest Inter-Database Compatibility and Discrimination

The PowerPlex® Fusion 6C System provides all of the materials needed for co-amplification and fluorescent detection of 27 loci, including the CODIS core loci and European Standard Set (ESS) loci, and Amelogenin and DYS391 for gender determination. The Penta D, Penta E, TPOX, D22S1045 and SE33 loci are also included. Finally, two rapidly mutating Y-STR loci, DYS570 and DYS576, are included in the multiplex. This comprehensive set supports compatibility with present databases across multiple regions and encompasses an expanded set of loci to address future growth. The PowerPlex® Fusion 6C System delivers more information and high success rates from demanding forensic, paternity and relationship-testing cases.

Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

High Discriminatory Power

The Probability of Identity value achieved with the PowerPlex® Fusion loci set is exceptional. By combining US CODIS and ESS loci, the system offers significant gains in discriminatory power and helps ensure fewer adventitious matches are obtained.

A probability of identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus.

STR Kit	Number of Loci	PI* (N = 1036)
GlobalFiler™ STR Kit	23	1.63 × 10^–27
Investigator® 24plex Kit	23	1.63 × 10^–27
PowerPlex® Fusion System	24	1.38 × 10^–28
PowerPlex® Fusion 6C System	27	9.09× 10^–31

*Note: PI values do not include Amelogenin or Y-STRs.

Reference: Steffen, C.R. et al. (2017) FSI Genetics Corrigendum to ‘U.S. Population Data for 29 Autosomal STR Loci’. doi: https://doi.org/10.1016/j.fsigen.2017.08.011

Superior Sensitivity

The PowerPlex® Fusion 6C System reliability produces complete STR profiles from as little as 125pg of human DNA and usable, partial profiles from DNA amounts lower than 125pg.

PowerPlex® Fusion 6C System

GlobalFiler® PCR Amplification Kit

Enhanced Inhibitor Tolerance

The PowerPlex® Fusion 6C System can successfully meet the challenges of inhibitor-laden samples, increasing your success rate and minimizing the need to re-amplify samples.

Direct-Amplification Compatibility for Most Sample Types

The PowerPlex® Fusion 6C System enables direct amplification with 12.5μl reactions from numerous sample types, including FTA® card punches, pretreated nonFTA cards (data not shown) and commonly used swabs. Laboratories can streamline their workflow while stretching their budget.

12562MA-W — Direct amplification of two 1.2mm punches from a buccal sample on an FTA® card using the recommended protocol for 12.5μl reactions.

FTA® Card Punches

12563MA-W — A buccal swab was pretreated with SwabSolution™ Reagent as recommended in the SwabSolution™ Kit Technical Manual. Following incubation, 2μl of extract was added to the PCR amplification mix using the recommended protocol for 12.5μl reactions.

Swab

Rapid Processing Solutions for PowerPlex® Systems

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® 21, 18D, 16HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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Which Product is Right for Me?

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Protocols

Complete Protocol

PowerPlex® Fusion 6C System for Use on the Spectrum Compact CE System Technical ManualPDF (17991 KB) – English

PowerPlex® Fusion 6C System for Use on the Applied Biosystems® Genetic Analyzers Technical ManualPDF (9628 KB) – English

PowerPlex® Fusion 6C System for Use on the Spectrum CE System Technical ManualPDF (5571 KB) – English

Quick Protocols

Use of the PowerPlex Fusion 6C System to Amplify Extracted DNA FB180PDF (203 KB) – English

Use of the PowerPlex Fusion 6C System to Amplify DNA from Swabs FB181PDF (191 KB) – English

Use of the PowerPlex Fusion 6C System to Amplify DNA from Storage Card Punches FB182PDF (197 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
PowerPlex® Fusion 6C Allelic Ladder Mix	DG417B	1 × 25μl
WEN Internal Lane Standard 500	DG500A	1 × 200μl		View Product
PowerPlex® Fusion 6C 5X Primer Pair Mix	DK419B	1 × 250μl
PowerPlex® Fusion 6C 5X Master Mix	DP114A	1 × 250μl
Water, Amplification Grade	DW099A	1 × 1,250μl		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-6C, CXR-6C, TOM-6C and WEN-6C dyes are proprietary.

What's in the box?

Item	Part #	Size	Concentration	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
PowerPlex® Fusion 6C Allelic Ladder Mix	DG417B	4 × 25μl
WEN Internal Lane Standard 500	DG500A	2 × 200μl		View Product
PowerPlex® Fusion 6C 5X Primer Pair Mix	DK419B	4 × 250μl
PowerPlex® Fusion 6C 5X Master Mix	DP114A	4 × 250μl
Water, Amplification Grade	DW099A	5 × 1,250μl		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-6C, CXR-6C, TOM-6C and WEN-6C dyes are proprietary.

What's in the box?

Item	Part #	Size	Concentration	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
PowerPlex® Fusion 6C Allelic Ladder Mix	DG417C	4 × 100μl
WEN Internal Lane Standard 500	DG500A	5 × 200μl		View Product
PowerPlex® Fusion 6C 5X Primer Pair Mix	DK419C	4 × 1,000μl
PowerPlex® Fusion 6C 5X Master Mix	DP114B	4 × 1,000μl
Water, Amplification Grade	DW099A	10 × 1,250μl		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-6C, CXR-6C, TOM-6C and WEN-6C dyes are proprietary.

Resources

Articles

Citations

Developmental validation of the PowerPlex® Fusion 6C System.

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2016 Forensic Sci. Int. Genet.
See all citations

PowerPlex® Fusion 6C System

6-Color Co-Amplification and Detection of 27 Loci

Highest Inter-Database Compatibility and Discrimination

High Discriminatory Power

A probability of identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus.

Superior Sensitivity

PowerPlex® Fusion 6C System

GlobalFiler® PCR Amplification Kit

Enhanced Inhibitor Tolerance

Direct-Amplification Compatibility for Most Sample Types

FTA® Card Punches

Swab

Rapid Processing Solutions for PowerPlex® Systems

Which Product is Right for Me?

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Resources

Articles

Citations

Software and Firmware

Posters

Other Resources

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PowerPlex® Fusion System

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