PowerPlex® Y23 System

Amplify 23 Y-STR Loci in a Single Reaction

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples. Higher power of discrimination from 23 loci results in fewer false-positive matches and more meaningful STR analysis. A single kit for both casework and databasing simplifies your workflow.

The PowerPlex® Y23 System allows co-amplification and four-color detection of 23 male-specific STR loci: DYS576, DYS389I/II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438 (penta), DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643 (penta), DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4.

For laboratories that store their plates up to 48 hours prior to injection, we recommend the use of the optional Stabilizer Reagent (Cat.# DM6571), sold separately.

New!

Learn how to interpret results from Y-STR DNA typing using the Y-Chromosome Haplotype Reference Database (YHRD), a free resource.

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Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Amplify Only Male DNA

The PowerPlex® Y23 System can successfully amplify trace amounts of male DNA, even in the presence of overabundant amounts of female DNA.

13065MA-W — Amplification of decreasing amounts of male DNA in the presence of 1µg of female DNA with two Y-STR kits: PowerPlex® Y23 System and Yfiler® Plus PCR Amplification Kit. Amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Obtain Full Profiles from the Most Challenging Samples

The PowerPlex® Y23 System is a robust multiplex that is tolerant of many amplification inhibitors, including hematin, tannic acid and humic acid. The robust performance of the kit results in more interpretable data and less need for re-amplification of inhibitor-laden samples.

13066MA-W — Amplification of 500pg of male DNA (PowerPlex® Y23 System) or 1.0ng of male DNA (Yfiler® Plus PCR Amplification Kit) in the presence of increasing amounts of common inhibitors. DNA was amplified for 30 cycles, and amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Streamline Workflows with Direct Amplification

The PowerPlex® Y23 System also is compatible with direct amplification, enabling streamlined Y-STR databasing efforts. Amplification can be successfully performed with sample types such as FTA® card punches as well as pretreated swabs, Bode Buccal DNA Collector™ punches or S&S 903 punches. Faster cycling conditions cut amplification time almost in half. Moreover, reduced sample-processing time and faster cycling conditions provide a significant time savings in every run.

Buccal Sample on FTA® Card

10433MA-W — Amplification of DNA from two 1.2mm punches from buccal cells on an FTA® card (direct amplification) using 27 cycles and the PowerPlex® Y23 System. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Swab Sample with SwabSolution™ Reagent

13067MA-W — Amplification of DNA from a cotton swab pretreated with SwabSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Buccal Sample with PunchSolution™ Reagent

13068MA-W — Amplification of one 1.2mm Bode Buccal DNA Collector™ punch preprocessed with the PunchSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Rapid Processing Solutions for PowerPlex® Systems

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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Protocols

Complete Protocol

PowerPlex® Y23 System for Use on the Applied Biosystems® Genetic Analyzers Technical ManualPDF (6779 KB) – English

PowerPlex® Y23 System for Use on the Spectrum CE System Technical ManualPDF (5366 KB) – English

PowerPlex® Y23 System for Use on the Spectrum Compact CE System Technical ManualPDF (14570 KB) – English

Quick Protocols

Use of the PowerPlex Y23 System to Amplify Extracted DNA FB159PDF (499 KB) – English

Use of the PowerPlex Y23 System to Amplify DNA from Swabs FB160PDF (500 KB) – English

Use of the PowerPlex Y23 System to Amplify DNA from Storage Card Punches FB161PDF (501 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
PowerPlex® Y23 5X Master Mix	DP108A	1 × 250μl
Water, Amplification Grade	DW099A	1 × 1,250μl		View Product
PowerPlex® Y23 Allelic Ladder Mix	DG248B	1 × 25μl
WEN Internal Lane Standard 500 Y23	DG520A	1 × 200μl		View Product
PowerPlex® Y23 10X Primer Pair Mix	DK209B	1 × 125μl

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

What's in the box?

Item	Part #	Size	Concentration	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
Water, Amplification Grade	DW099A	5 × 1,250μl		View Product
PowerPlex® Y23 Allelic Ladder Mix	DG248B	4 × 25μl
WEN Internal Lane Standard 500 Y23	DG520A	1 × 200μl		View Product
PowerPlex® Y23 10X Primer Pair Mix	DK209B	4 × 125μl
PowerPlex® Y23 5X Master Mix	DP108A	4 × 250μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

What's in the box?

Item	Part #	Size
Stabilizer Reagent	DM657A	1 × 0.25ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Citations

A substantially lower frequency of uninformative matches between 23 versus 17 Y-STR haplotypes in north Western Europe.

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2014 Forensic Sci. Int. Genet.
See all citations

Software and Firmware

PowerPlex® Systems Panels and Bins

PowerPlex® Y23 System

5-Dye STR Multiplex for Amplification of 23 Y-STR Loci

Amplify 23 Y-STR Loci in a Single Reaction

Amplify Only Male DNA

Obtain Full Profiles from the Most Challenging Samples