Lumit® VEGF-A (Human) Immunoassay

new-kit-box

ELISA Alternative for VEGF-A Detection

Please Enquire
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Lumit® VEGF-A (Human) Immunoassay

Quantify VEGF-A Release in Less Than 70 Minutes

Vascular Endothelial Growth Factor A (VEGF-A), a multifunctional cytokine, is important for normal development and the maintenance of the vascular and lymphatic systems. It is produced by many cells, including endothelial cells, macrophages and activated T cells. While VEGF-A plays a beneficial role in wound healing, its dysregulation contributes to tumorigenesis and vascular disease in various parts of the body.

The Lumit® VEGF-A Immunoassay quantitatively measures released VEGF-A in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer—the entire protocol is completed in less than 70 minutes. Use the assay directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® VEGF-A Immunoassay Works

Primary antibodies to VEGF-A were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of VEGF-A, the subunits come together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to VEGF-A levels. The assay recognizes human VEGF-A, but not mouse or rat VEGF-A. It is specific for VEGF-A (121, 165 and 183) and does not cross-react with VEGF-B, -C and -D family members.

Schematic showing Lumit® Immunoassay concept.

Avoid Tedious Wash Steps with Simple One-Plate Protocol

Lumit® Immunoassay Workflow

Broad Linear Range of Lumit® VEGF-A Detection

The picomolar to nanomolar linear detection range of the Lumit® VEGF-A Immunoassay covers expected concentrations for most cell models.

Graph showing standard curve for Lumit® VEGF-A (Human) Immunoassay.

Specification

Lumit® VEGF-A Immunoassay

Dynamic Range

18.2–25,000pg/ml

Limit of Detection

3pg/ml (3 SD above background)

Minimal Detectable Dose (MDD)

2pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Featured Webinar

Optimizing the Lumit® IL-6 Immunoassay for High-Throughput Drug Screening

In this webinar, we share how to scale and optimize Lumit® Immunoassays for HTS applications. Learn how researchers used the Lumit® IL-6 Immunoassay in a screening campaign for antiviral drugs.

View On-Demand Webinar

 

Need to measure a different target?

View our growing portfolio of
Lumit® Immunoassays.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

Similar Products

Lumit® IL-1β Human/Mouse Immunoassay

Quantifies inflammasome activation by measuring released IL-1β using a simple, no-wash protocol.

W6010, W6011, W6012, W7010, W7011, W7012

Lumit® IFN-γ (Human) Immunoassay

Quantitatively measures released IFN-γ in cell culture samples using a simple, no-wash protocol.

W6040, W6042, W6041

Lumit® Immunoassay Labeling Kit and Detection Reagents

Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.

VB2500, VB2010, VB2020, VB2030, VB4050, VB4060

Lumit® IL-17A (Human) Immunoassay

Quantitatively measure released IL-17A in cell culture samples using a simple, no-wash protocol completed in 70 minutes or less.

Frequently Used With

Lumit® IL-8 (Human) Immunoassay

Quantitatively measure IL-8 secretion in cell culture samples using a simple, no-wash protocol completed in 70 minutes or less.

Lumit® TNF-α (Human) Immunoassay

Quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol.

W6050, W6052, W6051

Lumit® IL-6 (Human) Immunoassay

Quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol.

W6030, W6032, W6031

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.