FuGENE® HD Transfection Reagent
Better Transfection for Challenging Cell Lines
- Difficult-to-transfect cell lines
- Transfection of primary cells
- Protein and virus production
Catalog Number:
Size
Transfect Cancer Model Cell Lines, Insect Cells and Stem Cells
Use the Best Cell Line for Your Study
FuGENE® HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al. [2009] Biotechniques 47, 617–624). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production.
FuGENE® HD gives more biologically relevant results by allowing you to use the best cell line for your study, and doesn’t require harsh treatment of the cells by lowering serum concentration or washing of toxic lipid complexes. The in vivo environment even can be mimicked with transfection in 100% serum.
Unrivaled Transfection Performance
Transfection of Stem Cells
“FuGENE® HD resulted in the highest stable transfection rate, estimated by antibiotic selection, with plasmids containing genes under the control of the EF1alpha or PGK promoters”.
Liu J., et al. (2009) Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol. Reprod. Dev. (2009) 76, 580-6.
Transfection of Cancer Cell Models
FuGENE® HD Transfection Reagent lets you work in your cancer model. Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line.
Cellular Imaging
High expression levels and the low toxicity of FuGENE® HD can bring better performance for imaging experiments. Read more about the low toxicity of FuGENE® HD in:
Antczuk et al. (2014) A High Content Assay to Assess Cellular Fitness. Comb Chem High Throughput Screen. 17(1), 12–24.
Protein Production
FuGENE® HD is made without animal products and is compatible with defined media. This means you can conduct experiments in mammalian and eukaryotic cells with confidence.
High Expression with Minimal Effect on Cell Health
This figure compares transfection reagent performance in HEK293 cells. Cells were transfected with a luciferase reporter plasmid, and luciferase activity detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. FuGENE® HD Transfection Reagent gave the highest expression levels and lowest toxicity.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
FuGENE® HD Transfection Reagent |
E231A | 1 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
FuGENE® HD Transfection Reagent |
E231A | 5 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FuGENE is a registered trademark of Fugent, L.L.C., USA.
FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.
Resources
Articles
- Choosing the Right Transfection Reagent for Optimal Efficiency
- Analyzing the Human Proteasome with Proteomics Techniques
- Discovery of an orally bioavailable and selective PKMYT1 inhibitor, RP-6306
- G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker, promotes proliferation and invasion of triple negative breast cancer
- An interaction between SLC35A1 and ST3Gal4 is differentially affected by CDG-causing mutations in the SLC35A1 gene
- Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
- In vitro structure–activity relationships and forensic case series of emerging 2‑benzylbenzimidazole ‘nitazene’ opioids
- Choosing the Best Transfection Reagent for Your Cell Line
- Multiplexing with NanoDLR and CellTiter-Fluor Assays
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Frequently Used With
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pmirGLO Dual-Luciferase miRNA Target Expression Vector
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Product Citations
Recent publications that mention the use of this product.
