ViaFect™ Transfection Reagent

Best Transfection Performance Across Many Cell Lines

Performs better than or equal to other best-in-class transfection reagents
Simple transfection protocol with minimal optimization required
Low toxicity for routine and challenging cell lines

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0.75ml 2 × 0.75ml

Catalog number selected: E4981

$ 416.00

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ViaFect™ Transfection Reagent

0.75ml

$ 416.00

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Develop More Biologically Relevant Assays. You Pick the Cell Line.

Cell biology is critical to transfection-based experiments. Not only do the cells need to maintain their viability and metabolism through the transfection process, they also must accurately represent the biology being modeled in the experiment. ViaFect™ Transfection Reagent allows high-efficiency transfection of a wide range of cell types without compromising cell viability, and provides an easy-to-use protocol that gives superior performance with minimal optimization. Now you can design the right assay in the right cells to model the biology you are studying.

Excellent Performance Compared With Other Best-in Class Transfection Reagents

Promega offers 3 premium transfection reagents, FuGENE® 6, FuGENE® HD and ViaFect. All offer broad-spectrum transfection capability and low toxicity. We performed transfection experiments with FuGENE® 6, FuGENE® HD, ViaFect and Lipofectamine (ThermoFisher Scientific). Although each reagent clearly worked best on certain cell lines, and none were perfect for every cell line, ViaFect™ Transfection Reagent yielded equal or greater luciferase expression over a wider variety of cell lines than any of the other reagents tested.

Full details of these transfection experiments are available in the article below:

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Cell Line	ViaFect	FuGENE® 6	FuGENE® HD	Lipofectamine® 2000	Compare ViaFect, FuGENE, Lipofectamine® 2000	Compare ViaFect, Lipofectamine® 3000
A549	+++	++	+++		See Data	See Data
C2C12	+++				See Data	See Data
CHO	+++	++		++	See Data
COS7	+++	+++	+++	+++	See Data
H9C2	+++			++	See Data
HCT116	+++		+++	++	See Data	See Data
HEK293	+++	++			See Data	See Data
HeLa	++		+++		See Data
HepG2	+++				See Data	See Data
HT-29	+++			+++	See Data
Jurkat	+++			++	See Data	See Data
K562				+++	See Data	See Data
LNCaP	+++				See Data
MCF7	+++			+++	See Data	See Data
NIH-3T3	++			+++	See Data
PC-12	+++		++	+++	See Data
PC-3	+++		+++		See Data	See Data
RAW 264.7		+++	+++	++	See Data
U2OS	+++		++		See Data	See Data
Key +++ = >80% of maximum RLU (Relative Luminescence Units); ++ = 50–80% of maximum RLU Each cell line was transfected with a luciferase reporter plasmid using either ViaFect™ Transfection Reagent, Lipofectamine® 2000 Transfection Reagent or Lipofectamine® 3000 Transfection Reagent following manufacturer instructions, applying either 5µl (50ng of DNA) or 10µl (100ng of DNA) of the transfection complex in each well of a 96-well plate. Luciferase expression (bars) and cell viability (lines) were measured either 24h post-transfection (Lipofectamine® 2000 comparison) or 24hr and 48hr post-transfection (Lipofectamine® 3000 comparison) using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay.

Transfect the Cell Line of Your Choice

Design biologically relevant assays in workhorse adherent cell models, suspension cells important for studying cell signaling, and even differentiated cell lines derived from stem cells. ViaFect™ Transfection Reagent gives you the flexibility to do the research you need to do.

iCell hepatocytes transfected with viafect reagent 12642-W — iCell® Hepatocytes*

iCell® Hepatocytes transfected with ViaFect™ Transfection Reagent and a GFP reporter plasmid at a 6:1 reagent:DNA ratio; GFP expression imaged one day post-transfection.

cardiomyocytes transfected with viafect reagent 12643-W — iCell® Cardiomyocytes*

iCell® Cardiomyocytes transfected with ViaFect™ Transfection Reagent and a GFP reporter plasmid at a 2:1 reagent:DNA ratio; GFP expression imaged one day post-transfection.

Neurons transfected with Viafect reagent 12644-W — iCell® DopaNeurons*

iCell® DopaNeurons transfected with ViaFect™ Transfection Reagent and a GFP reporter plasmid at a 4:1 reagent:DNA ratio; GFP expression imaged 3 days post-transfection.

*Experiments were performed using Cellular Dynamics iCell® human tissue cells plated into 96 well plates. Data courtesy of Cellular Dynamics International.

ViaFect™ Transfection Citations, Cell Line by Cell Line

ViaFect™ Transfection Reagent is gaining popularity as a high-performance, low-toxicity reagent that performs well across a wide range of cell lines. Expand the section below to see a list of 2018 and 2017 articles citing use of ViaFect in a variety of cell types.

Expand to View Citations

Here are over 100 papers from 2017 and 2018 demonstrating use of ViaFect with many different cell types. Enter your cell line of interest in the Search field to see if it is represented in this collection.

Search citations

Cell Line	Citation
A549	Somensi, N., et al. (2017) Extracellular HSP70 Activates ERK1/2, NF-kB and Pro-Inflammatory Gene Transcription Through Binding with RAGE in A549 Human Lung Cancer Cells. Cell. Physiol. Biochem. 42, 2507-22.
AF22	Chai, M., et al. (2018) Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors. Genes Dev. 32, 165-80.
AGS	Cerda-Opazo, P., et al. (2018) Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer. Oncotarget 9, 12853-67.
AU565	Bagu, E.T., et al. (2017) Repression of Fyn-related kinase in breast cancer cells is associated with promoter site-specific CpG methylation. Oncotarget 8, 11442-59.
B16F0	Morita, T. and Hayashi, K. (2018) Tumor progression is mediated by thymosin-β4 through a TGFβ/MRTF signaling axis. Mol. Cancer Res. 16, 880-93.
B16F1	Morita, T. and Hayashi, K. (2018) Tumor progression is mediated by thymosin-β4 through a TGFβ/MRTF signaling axis. Mol. Cancer Res. 16, 880-93.
BJ1-7TGP	Kramer, N., et al. (2017) Autocrine WNT2 signaling in fibroblasts promotes colorectal cancer progression. Oncogene 36, 5460-72.
Bovine Aortic Endothelial Cells	Gupta, R.M., et al. (2017) A genetic variant associated with five vascular diseases is a distal regulator of endothelin-1 gene expression. Cell 170, 522-33.
BT	Alkheraif, A.A., et al. (2017) Type 2 BVDV Npro suppresses IFN-1 pathway signaling in bovine cells and augments BRSV replication. Virology 507, 123-34.
C2C12	Saklayen, N., et al. (2017) Analysis of poration-induced changes in cells from laser-activated plasmonic substrates. Biomed. Opt. Express 8, 4756-71.
CHO	Hoonakker, M.E., et al. (2017) Reporter cell lines for detection of pertussis toxin in acellular pertussis vaccines as a functional animal-free alternative to the in vivo histamine sensitization test. Vaccine 35, 1152-60.
COS-7	Nakagawa, T., et al. (2017) N-glycan-dependent cell-surface expression of the P2Y2 receptor and N-glycan-independent distribution to lipid rafts. Biochem. Biophys. Res. Comm. 485, 427-31.
COV-504	Lu, T., et al. (2018) Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Sci. 109, 2221-34.
EH-GB1	Ma, Q., et al. (2018) EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer Lett. 430, 97-108.
EH-GB1	Hao, J., et al. (2017) Downregulation of BRD4 inhibits gallbladder cancer proliferation and metastasis and induces apoptosis via PI3K/AKT pathway. Int. J. Oncol. 51, 823-32
GBC-SD	Ma, Q., et al. (2018) EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer Lett. 430, 97-108.
GBC-SD	Hao, J., et al. (2017) Downregulation of BRD4 inhibits gallbladder cancer proliferation and metastasis and induces apoptosis via PI3K/AKT pathway. Int. J. Oncol. 51, 823-33
GIST882	Wang, L., et al.(2017) Orai1 mediates tumor-promoting store-operated Ca2+ entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal–regulated kinase pathway. Tumor Biol. 39, 1010428317691426.
H1299	Eriksson, M., et al. (2017) The effect of mutant p53 proteins on glycolysis and mitochondrial metabolism. Mol. Cell. Biol. 37, e00328-17.
H358	Motooka, Y., et al. (2017) Pathobiology of Notch2 in lung cancer. Pathology 49, 486-93.
H5 Mouse chondrocytes	Kalev-Zylinska, M.L., et al. (2018) Altered N-methyl D-aspartate receptor subunit expression causes changes to the circadian clock and cell phenotype in osteoarthritic chondrocytes. Osteoarthr. Cartilage https://doi.org/10.1016/j.joca.2018.06.015
H9c2	Mudaliar, H, et al. (2017) Remote ischemic preconditioning attenuates EGR-1 expression following myocardial ischemia reperfusion injury through activation of the JAK-STAT pathway. Int. J. Cardiol. 228, 729-41.
HCC	Xu, S., et al. (2018) NFATc1 is a tumor suppressor in hepatocellular carcinoma and induces tumor cell apoptosis by activating the FasL-mediated extrinsic signaling pathway. Cancer Med. DOI: 10.1002/cam4.1716.
HCC1569	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
HCC70	Bagu, E.T., et al. (2017) Repression of Fyn-related kinase in breast cancer cells is associated with promoter site-specific CpG methylation. Oncotarget 8, 11442-59.
HCT 116	Kranz, P., et al. (2017) PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR). Cell Death Dis. 8, e2986.
HCT 116	Lin, H., et al. (2017) DHX32 promotes angiogenesis in colorectal cancer through augmenting β-catenin signaling to induce expression of VEGFA. EBioMedicine 18, 62-72
HCT 116	Eriksson, M., et al. (2017) The effect of mutant p53 proteins on glycolysis and mitochondrial metabolism. Mol. Cell. Biol. 37, e00328-17.
HCT-8	Lin, H., et al. (2017) DHX32 promotes angiogenesis in colorectal cancer through augmenting β-catenin signaling to induce expression of VEGFA. EBioMedicine 18, 62-72
HEK 293	Higashioki, K., et al. (2017) Myogenic differentiation from MYOGENIN-mutated human iPS cells by CRISPR/Cas9. Stem Cells Intl. 9210494.
HEK 293T	Foxler, D.E., et al. (2018) A HIF–LIMD1 negative feedback mechanism mitigates the pro‐tumorigenic effects of hypoxia. EMBO Mol. Med. 10, e8304
HEK 293T	Zhang, Y., et al. (2017) Rescue of Pink1 deficiency by stress-dependent activation of autophagy. Cell Chem. Biol. 24, 471-80.
HEK 293T	Cerda-Opazo, P., et al. (2018) Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer. Oncotarget 9, 12853-67.
HeLa	Monson, E.A., et al. (2018) Lipid droplet density alters the early innate immune response to viral infection. PLoS One 13, e0190597.
HeLa	Foxler, D.E., et al. (2018) A HIF–LIMD1 negative feedback mechanism mitigates the pro‐tumorigenic effects of hypoxia. EMBO Mol. Med. 10, e8304.
HeLa	Jeong, M., et al. (2017) USP8 suppresses death receptor-mediated apoptosis by enhancing FLIPL stability. Oncogene 36, 458-70.
HeLa	Heschen, C.L., et al. (2017) NuMA recruits dynein activity to microtubule minus-ends at mitosis. eLife 6, e29328.
HepG2	Qi, Z., et al. (2017) Asiatic acid enhances Nrf2 signaling to protect HepG2 cells from oxidative damage through Akt and ERK activation. Biomed. Pharmacother. 88, 252-9
HepG2	Xu, H., et al. (2018) MicroRNA-122 supports robust innate immunity in hepatocytes by suppressing STAT3 phosphorylation. bioRχiv doi: http://dx.doi.org/10.1101/250748
Hs578T	Oh, S., et al.(2017) Silencing of Glut1 induces chemoresistance via modulation of Akt/GSK-3β/β-catenin/survivin signaling pathway in breast cancer cells. Arch. Biochem. Biophys. 636, 110-22.
Hs578T	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
Hu5/KD3	Higashioki, K., et al. (2017) Myogenic differentiation from MYOGENIN-mutated human iPS cells by CRISPR/Cas9. Stem Cells Intl. 9210494
HuH-7	Monson, E.A., et al. (2018) Lipid droplet density alters the early innate immune response to viral infection. PLoS One 13, e0190597.
HuH-7	Xu, H., et al. (2018) MicroRNA-122 supports robust innate immunity in hepatocytes by suppressing STAT3 phosphorylation. bioRχiv doi: http://dx.doi.org/10.1101/250747
HuH-7.5	Eyre, N.S., et al. (2017) Sensitive luminescent reporter viruses reveal appreciable release of hepatitis C virus NS5A protein into the extracellular environment. Virology 507, 20-31.
Human primary cumulus cells	Qiao, G., et al. (2017) Deregulation of WNT2/FZD3/β-catenin pathway compromises the estrogen synthesis in cumulus cells from patients with polycystic ovary syndrome. Biochem. Biophys. Res. Comm. 493, 847-54.
Human foreskin fibroblasts	Kalser, J., et al. (2017) Differences in growth properties among two human cytomegalovirus glycoprotein O genotypes. Front. Microbiol. 8, 1609.
Human podocytes	Fan, Y., et al. (2017) Rtn1a-mediated endoplasmic reticulum stress in podocyte injury and diabetic nephropathy. Sci. Reports 7, 323.
Human pulmonary artery endothelial cells (HPAEC)	Nakajima, H., et al. (2017) Flow-dependent endothelial YAP regulation contributes to vessel maintenance. Develop. Cell 40, 523-36.
Human small airway epithelial cells (SAEC)	Mao, P., et al. (2017) MicroRNA-19b mediates lung epithelial-mesenchymal transition via phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase in response to mechanical stretch. Am. J. Resp. Cell Mol. Biol. 56, 11-9.
Human synovial fluid-derived mesenchymal stem cells	Liu, W., et al. (2017) IL-1β impedes the chondrogenic differentiation of synovial fluid mesenchymal stem cells in the human temporomandibular joint. Int. J. Mol. Med. 39, 317-26.
Human umbilical vein endothelial cells	Wang, J., et al. (2017) NINJ2– A novel regulator of endothelial inflammation and activation. Cell Signal. 35, 231-41.
ICC	Zhu, X., et al. (2017) Osthole induces apoptosis and suppresses proliferation via the PI3K/Akt pathway in intrahepatic cholangiocarcinoma. Int. J. Mol. Med. 40, 1143-51.
iCell® cardiomyocytes	Hall, A.R., et al. (2018) Visualizing mutation-specific differences in the trafficking-deficient phenotype of Kv11.1 proteins linked to long QT syndrome type 2. Front. Physiol. 9, 284.
iCell® cardiomyocytes	Fenix, A.M., et al. (2017) Muscle specific stress fibers give rise to sarcomeres and are mechanistically distinct from stress fibers in non-muscle cell. bioRχiv doi: https://doi.org/10.1101/235424.
iCell® cardiomyocytes	Taneja, N., et al. (2018) Focal adhesion kinase regulates early steps of myofibrillogenesis in cardiomyocytes. bioRχiv ; doi: http://dx.doi.org/10.1101/261248.
IGROV1	Alam, S., et al. (2017) Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells. Sci. Reports 7, 45367.
Induced hepatocyte-like cells (iHeps)	Katayama, H., et al. (2017) Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon. Sci. Reports 7, 44498.
INS-1	Spohrer, S., et al. (2017) Functional interplay between the transcription factors USF1 and PDX-1 and protein kinase CK2 in pancreatic β-cells. Sci. Reports 7, 16367
K562	Espadinha, A.-S., et al. (2017) A tyrosine kinase-STAT5-miR21-PDCD4 regulatory axis in chronic and acute myeloid leukemia cells. Oncotarget 8, 76174-88.
MCF-10A	Eriksson, M., et al. (2017) The effect of mutant p53 proteins on glycolysis and mitochondrial metabolism. Mol. Cell. Biol. 37, e00328-17.
MCF7	Bruno, W., et al. (2017) Functional analysis of a CDKN2A 5'UTR germline variant associated with pancreatic cancer development. PLoS One 12, e0189123.
MCF7	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
MDA-MB-231	Oh, S., et al. (2017) Silencing of Glut1 induces chemoresistance via modulation of Akt/GSK-3β/β-catenin/survivin signaling pathway in breast cancer cells. Arch. Biochem. Biophys. 636, 110-22.
MDA-MB-231	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
MDA-MB-231	Eriksson, M., et al. (2017) The effect of mutant p53 proteins on glycolysis and mitochondrial metabolism. Mol. Cell. Biol. 37, e00328-17.
MDA-MB-435s	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
MDA-MB-436	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
MDA-MB-438	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
MIN6-K8	Zhang, W., et al. (2017) Neuromedin U suppresses glucose-stimulated insulin secretion in pancreatic β cells. Biochem. Biophys. Res. Comm. 493, 677-83.
MIO-M1	Chu-Tan, J.A., et al. (2018) MicroRNA-124 dysregulation is associated with retinal inflammation and photoreceptor death in the degenerating retina. Invest. Ophthalmol. Vis. Sci. 59, 4094-105.
MOE	Dean, M., Davis, D.A. and Burdette, J.E. (2017) Activin A stimulates migration of the fallopian tube epithelium, an origin of high-grade serous ovarian cancer, through non-canonical signaling. Cancer Lett. 391, 114-24.
Mouse embryonic fibroblast	Campostrini, G., et al. (2017) The expression of the rare caveolin-3 variant T78M alters cardiac ion channels function and membrane excitability. Cardiovasc. Res. 113, 1256-65.
Mouse Oligodendrocyte progenitor cells (mOPC)	Weider, M., et al. (2018) Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning. Nat. Comm. 9, 899.
MRC4V1	Bertoletti, F., et al. (2017) Phosphorylation regulates human polη stability and damage bypass throughout the cell cycle. Nucl. Acids Res. 45, 9441-54.
Murine neural stem cells	Bansod, S., Kageyama, R., and Ohtsuka, T. (2017) Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development. Development 144, 3156-67.
Murine renal tubular epithelial cells	Yuan, X., et al. (2017) Bone mesenchymal stem cells ameliorate ischemia/reperfusion-induced damage in renal epithelial cells via microRNA-223. Stem Cell Res. Ther. 8, 146
NCCIT	Park, S.-W., et al. (2017) NANOG gene expression is regulated by the ETS transcription factor ETV4 in human embryonic carcinoma NCCIT cells. Biochem. Biophys. Res. Comm.487, 532-8.
NCI-N87	Cerda-Opazo, P., et al. (2018) Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer. Oncotarget 9, 12853-67.
NIH 3T3	Bansod, S., Kageyama, R., and Ohtsuka, T. (2017) Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development. Development 144, 3156-67.
NIT-1	Lee, J.-J., et al. (2017) Calcium ion induced structural changes promote dimerization of secretagogin, which is required for its insulin secretory function. Sci. Reports 7, 6976.
NMuMG	Morita, T. and Hayashi, K. (2018) Tumor progression is mediated by thymosin-β4 through a TGFβ/MRTF signaling axis. Mol. Cancer Res. 16, 880-93.
NOZ	Ma, Q., et al. (2018) EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer Lett. 430, 97-108.
NOZ	Hao, J., et al. (2017) Downregulation of BRD4 inhibits gallbladder cancer proliferation and metastasis and induces apoptosis via PI3K/AKT pathway. Int. J. Oncol. 51, 823-31
OCUG	Ma, Q., et al. (2018) EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer Lett. 430, 97-108.
OCUG	Hao, J., et al. (2017) Downregulation of BRD4 inhibits gallbladder cancer proliferation and metastasis and induces apoptosis via PI3K/AKT pathway. Int. J. Oncol. 51, 823-35
PANC1	Villarino, N., et al. (2017) A screen for inducers of bHLH activity identifies pitavastatin as a regulator of p21, Rb phosphorylation and E2F target gene expression in pancreatic cancer. Oncotarget 8, 53154-67.
PBMC	Cai, F., et al. (2018) MicroRNA-146b-3p regulates the development and progression of cerebral infarction with diabetes through RAF1/P38MAPK/COX-2 signaling pathway. Am. J. Transl. Res. 10, 618-28.
PC3	Xu, H., et al. (2018) MicroRNA-122 supports robust innate immunity in hepatocytes by suppressing STAT3 phosphorylation. bioRχiv doi: http://dx.doi.org/10.1101/250746
PtK2	Long, A.F., Udy, D.B., and Dumont, S. (2017) Hec1 tail phosphorylation differentially regulates mammalian kinetochore coupling to polymerizing and depolymerizing microtubules. Curr. Biol. 27, 1692-9.
PtK2	Heschen, C.L., et al. (2017) NuMA recruits dynein activity to microtubule minus-ends at mitosis. eLife 6, e29328.
RAW264.7	Ricci, E., et al. (2017) Role of the glucocorticoid-induced leucine zipper gene in dexamethasone-induced inhibition of mouse neutrophil migration via control of annexin A1 expression. FASEB J. 31, 3054-65.
RMC	Gao, H.-Y. and Han, C.-X. (2017) The role of PTEN up-regulation in suppressing glomerular mesangial cells proliferation and nephritis pathogenesis. Eur. Rev. Med. Pharmacol. Sci. 21, 3634-41.
RPE1	Heschen, C.L., et al. (2017) NuMA recruits dynein activity to microtubule minus-ends at mitosis. eLife 6, e29328.
SaOS2	Nishita, M., et al. (2017) Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Sci. Reports 7, 1.
SGC-996	Ma, Q., et al. (2018) EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer Lett. 430, 97-108.
SGC-996	Hao, J., et al. (2017) Downregulation of BRD4 inhibits gallbladder cancer proliferation and metastasis and induces apoptosis via PI3K/AKT pathway. Int. J. Oncol. 51, 823-34
SH-SY5Y	Zhang, Y., et al. (2017) Rescue of Pink1 deficiency by stress-dependent activation of autophagy. Cell Chem. Biol. 24, 471-80.
SK-BR-3	Onodera, Y., et al. (2018) Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe. Nat. Comm. 9, 2682.
SK-HEP-1	McDonald, C.J., et al. (2018) Evaluation of a bone morphogenetic protein 6 variant as a cause of iron loading. Hum. Genom. 12, 23.
SKOV3	Yan, X.-Y., et al. (2017) p62/SQSTM1 as an oncotarget mediates cisplatin resistance through activating RIP1-NF-κB pathway in human ovarian cancer cells. Cancer Sci. 108, 1405-13.
SKOV3	Lu, T., et al. (2018) Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Sci. 109, 2221-34.
SKOV3	Alam, S., et al. (2017) Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells. Sci. Reports 7, 45367.
SNU-1	Cerda-Opazo, P., et al. (2018) Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer. Oncotarget 9, 12853-67
SW480	Lin, H., et al. (2017) DHX32 promotes angiogenesis in colorectal cancer through augmenting β-catenin signaling to induce expression of VEGFA. EBioMedicine 18, 62-72
SW620	Lin, H., et al. (2017) DHX32 promotes angiogenesis in colorectal cancer through augmenting β-catenin signaling to induce expression of VEGFA. EBioMedicine 18, 62-72
THP-1	Wiśnik, E., Płoszaj, T. and Robaszkiewicz, A. (2017) Downregulation of PARP1 transcription by promoter-associated E2F4-RBL2-HDAC1-BRM complex contributes to repression of pluripotency stem cell factors in human monocytes. Sci. Reports 7, 9483.
TT	Jang, S., et al. (2017) Histone deacetylase inhibitor thailandepsin-A activates Notch signaling and suppresses neuroendocrine cancer cell growth in vivo. Oncotarget 8, 70828-40.
U2OS	Foxler, D.E., et al. (2018) A HIF–LIMD1 negative feedback mechanism mitigates the pro‐tumorigenic effects of hypoxia. EMBO Mol. Med. 10, e8304.
U2OS	Nishita, M., et al. (2017) Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Sci. Reports 7, 1.
U373MG	Kwon, R.-J., et al.(2017) Roles of zinc-fingers and homeoboxes 1 during the proliferation, migration, and invasion of glioblastoma cells. Tumor Biol. 39, 1010428317694575.
U87	Diplas, B.H., et al. (2018) The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma. Nat. Comm. 9, 2087.
UACC-62	Jeong, M., et al. (2017) USP8 suppresses death receptor-mediated apoptosis by enhancing FLIPL stability. Oncogene 36, 458-70.
WI-38	Eriksson, M., et al. (2017) The effect of mutant p53 proteins on glycolysis and mitochondrial metabolism. Mol. Cell. Biol. 37, e00328-17.

Create Assays Not Possible With Other Reagents

Assay for cytokine signaling in a hematopoietic cell model.

TF-1 suspension cells were transiently transfected with pGL4.32[luc2P/NF-κB-RE/Hygro] Vector, an NF- κB response element luciferase reporter, using either ViaFect™ Transfection Reagent at a 2:1 reagent:DNA ratio or Amaxa Nucleofector® II (electroporation). The following day cells were stimulated with TNFα for 6 hours, and the response was measured with Bio-Glo™ Luciferase Reagent.

TF-1 cells transfected with viafect reagent 12105MB-W

Directly assay HIF1α protein stability in iPS-derived cardiomyocytes.

iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using pNLF1-HIF1A [CMV/neo] Vector, a HIF1A-NanoLuc® Luciferase fusion reporter, at a 4:1 reagent:DNA ratio. Twenty-four hours post-transfection cells were treated as indicated with hypoxia mimetics for 3 hours, and the amount of HIF1α-NanoLuc® protein was detected using Nano-Glo™ Luciferase Assay Reagent.

iPS Cardiomyocytes transfected with viafect 12106MB-W

Assay for hypoxia transcriptional response in iPS-derived cardiomyocytes.

iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using the pGL4.42[luc2P/HRE/Hygro] Vector, a hypoxia response element luciferase reporter, at a 4:1 reagent:DNA ratio. Twenty-four hours post-transfection, cells were treated with hypoxia mimetics as indicated for 6 hours followed by luciferase detection using ONE-Glo™ Luciferase Assay System.

cardiomyocyte transfection with viafect reagent 12107MB-W

Protocols

Complete Protocol

ViaFect™ Transfection Reagent Technical ManualPDF (1312 KB) – English

Quick Protocols

ViaFect Transfection Reagent FB165PDF (191 KB) – English

What's in the box?

Item	Part #	Size
ViaFect™ Transfection Reagent	E498A	1 × 0.75ml

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Do not freeze.

Patents and Disclaimers

ViaFect™ Transfection Reagent is sold with a Limited Use Label License.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product for research use only and in accordance with all applicable laws, rules and regulations. Researchers shall have no right to reverse engineer this product. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

What's in the box?

Item	Part #	Size
ViaFect™ Transfection Reagent	E498A	2 × 0.75ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

ViaFect™ Transfection Reagent

Best Transfection Performance Across Many Cell Lines

Develop More Biologically Relevant Assays. You Pick the Cell Line.

Excellent Performance Compared With Other Best-in Class Transfection Reagents

Transfect the Cell Line of Your Choice

ViaFect™ Transfection Citations, Cell Line by Cell Line

Create Assays Not Possible With Other Reagents

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Resources

Articles

Citations

Other Resources

ViaFect™ Transfection Reagent

Best Transfection Performance Across Many Cell Lines

Develop More Biologically Relevant Assays. You Pick the Cell Line.

Excellent Performance Compared With Other Best-in Class Transfection Reagents

Viafect™ Transfection Performance in A549 Cells

Viafect™ Transfection Performance in C2C12 Cells

Viafect™ Transfection Performance in CHO Cells

Viafect™ Transfection Performance in COS7 Cells

Viafect™ Transfection Performance in H9C2 Cells

Viafect™ Transfection Performance in HCT116 Cells

Viafect™ Transfection Performance in HEK293 Cells

Viafect™ Transfection Performance in HeLa Cells

Viafect™ Transfection Performance in HepG2 Cells

Viafect™ Transfection Performance in HT-29 Cells

Viafect™ Transfection Performance in Jurkat Cells

Viafect™ Transfection Performance in K562 Cells

Viafect™ Transfection Performance in LNcaP Cells

Viafect™ Transfection Performance in MCF7 Cells

Viafect™ Transfection Performance in NIH 3T3 Cells

Viafect™ Transfection Performance in PC-3 Cells

Viafect™ Transfection Performance in PC-12 Cells

Viafect™ Transfection Performance in RAW264.7 Cells

Viafect™ Transfection Performance in U2OS Cells

Transfect the Cell Line of Your Choice

ViaFect™ Transfection Citations, Cell Line by Cell Line

Create Assays Not Possible With Other Reagents

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Resources

Articles

Citations

Other Resources

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