Trypsin/Lys-C Mix, Mass Spec Grade

V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
Protein Cleavage with Trypsin-Lys-C
V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
customize-this-small
View information on global supply logistics

Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

$ 77.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Trypsin/Lys-C Mix, Mass Spec Grade
20μg
$ 77.00
Your price: Log in
Change Configuration

Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Trypsin digest illustration.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protocols

Complete Protocol

Specifications

You are viewing: V5071 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 1 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5072 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507B 1 × 100μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5073 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 5 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Posters

Other Resources

Similar Products

6 × 5 LC-MS/MS Peptide Reference Mix

Peptide mix and free software to monitor LC/MS instrument performance.

V7491, V7495

Rapid-Digestion Trypsin and Trypsin/Lys-C Kits

Streamlined protocol for fast, efficient protein digestion.

VA1060, VA1061

rAsp-N, Mass Spec Grade

Cleavage site: N-terminal of Asp. Optimal pH: 6–9.

VA1160

Frequently Used With

Arg-C, Sequencing Grade

Cleavage site: C-terminal of Arg. Optimal pH: 7.6–7.9.

V1881

Asp-N, Sequencing Grade

Cleavage site: N-terminal of Asp. Optimal pH: 4–9.

V1621

Chymotrypsin, Sequencing Grade

Cleavage sites: C-terminal of Tyr, Phe, Trp. Optimal pH: 7–9.

V1061, V1062

Let's find the product that meets your needs.

Talk to a Scientist

Jan

Jan

Germany
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.