Trypsin Gold, Mass Spectrometry Grade

V5280_Trypsin-Gold-Mass-Spec-Grade--100ug_3
customize-this-small
View information on global supply logistics

Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion

  • Each lot qualified by mass spectrometry to ensures compatibility with your applications/instrumentation
  • Chymotrypsin activity eliminated for distinct, consistent data
  • Digest in gel, or in solution

Size

Catalog number selected: V5280

$ 125.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Trypsin Gold, Mass Spectrometry Grade
100μg
$ 125.00
Your price: Log in

Excellent Specificity and Resistance to Autolytic Digestion

Trypsin Gold, Mass Spectrometry Grade, is manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.

Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel protein digestion and mass spectrometric analysis. For more information, see the Protein Purification and Analysis chapter of the Protocols and Applications Guide.

See our newest trypsin: Rapid Digestion-Trypsin and Rapid Digestion-Trypsin/Lys-C

Learn more about our custom options for this product at: www.promega.com/custom/

Spectrogram Showing Digestion with Trypsin Gold, Mass Spec Grade

Spectrogram of bovine carbonic anhydrase II protein digestion with Trypsin Gold, Mass Spectrometry Grade.

A 500ng sample of carbonic anhydrase II was separated by gel electrophoresis and digested with 500ng of Trypsin Gold, Mass Spectrometry Grade, overnight at 37°C.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

Trypsin Gold, Mass Spectrometry Grade

 V528A 1 × 100μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

Other Resources

Similar Products

Trypsin Platinum, Mass Spectrometry Grade

Maximum digest specificity. Maximum resistance to autoproteolysis.

VA9000

Trypsin/Lys-C Mix, Mass Spec Grade

Increased peptide recovery, enhanced protein quantitation, improved reproducibility.

V5071, V5072, V5073

IdeS and IdeZ Proteases

IgG-degrading enzymes that cleave at a single site below the hinge region.

V7511, V7515, V8341, V8345

Rapid-Digestion Trypsin and Trypsin/Lys-C Kits

Streamlined protocol for fast, efficient protein digestion.

VA1060, VA1061

AccuMAP® Low pH Protein Digestion Kits

Protein digestion at low (mildly acidic) pH to suppress deamidation and disulfide bond scrambling.

VA1040, VA1050

Frequently Used With

Arg-C Ultra, Mass Spec Grade

Endopeptidase that cleaves at the C-terminus of arginine residues, including Arg-Pro.

VA1831, VA1832

Lys-C, Mass Spec Grade

Cleavage site: C-terminal of Lys. Optimal pH: 7–9.

VA1170

Asp-N, Sequencing Grade

Cleavage site: N-terminal of Asp. Optimal pH: 4–9.

V1621

Glu-C, Sequencing Grade

Cleavage site: C-terminal of Glu. Optimal pH: 4–9.

V1651

rLys-C, Mass Spec Grade

Cleavage site: C-terminal of Lys. Optimal pH: 8–9.

V1671

Let's find the product that meets your needs.

Talk to a Scientist

Chris

Chris

US
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.