LMR MSI Analysis System

Resolve Microsatellite Instability Status in Challenging Sample Types

The LMR MSI Analysis System is a PCR-based method for detecting microsatellite instability (MSI) in solid tumors that will enable researchers to more precisely assess MSI status of their samples. This improved clarity is ideal for challenging samples with subtle MSI phenotypes, including those with ambiguous results or small shifts.

MSI applications include analysis of solid tumors, lynch syndrome samples and immuno-oncology research and

Increase MSI Detection Ability with Long Mononucleotide Repeat Markers

The new LMR panel is comprised of four Promega gold standard MSI markers (BAT-25, BAT-26, MONO-27 and NR-21) and four additional markers with increased repeat length (BAT-52, BAT-56, BAT-59 and BAT-60). This combination of markers provides increased MSI detection ability in difficult samples while maintaining overlap with data gathered using traditional markers. The assay has been optimized to enable strong and balanced amplification of all markers, even from challenging samples such as FFPE tissue.

Validation of LMR Markers for MSI Detection

A recent paper published by Johns Hopkins details how a research team, led by Dr. James R. Eshleman, conducted a validation of the LMR MSI Analysis System using a variety of tumor samples with previously confirmed dMMR/MSI-H status. Additionally, the study showed that the LMR markers demonstrated improved sensitivity for tumor samples that had inconclusive results in previous MSI testing. The study was supported in part by the Promega Clinical Research Program (PCRP) and is the first publication to result from a PCRP partnership.

Read the Paper on PubMed

LMR MSI Analysis Workflow

In a routine research laboratory, the time from sample input to MSI status determination can range from overnight to two days. With the LMR MSI Analysis System, you can have data ready for analysis overnight.

DNA Isolation

5 hours including incubation

Maxwell® RSC Instruments and Maxwell® RSC FFPE DNA Kit

Amplification

1+ hours

MSI Workflow: Microsatellite instability markers amplified

LMR MSI Analysis System

Capillary Electrophoresis

30 minutes

Promega Spectrum Compact or Applied Biosystems® 3500 Genetic Analyzer

Data Analysis

<1 hour

Customer CE Analysis Software

Explore additional MSI products and resources.

Clear and Confident Assessment of MSI Status

MSI analysis typically involves comparing allelic profiles of microsatellite markers generated by amplifying matched pairs of tumor and normal samples. The presence of alleles in the tumor sample that are not found in the matched normal sample are indicative of MSI.

An endometrial cancer tumor sample (Panels A–D) and matched normal sample (Panels E–H) were amplified using the LMR MSI Analysis System. Fragment analysis was performed using an Applied Biosystems® 3500 Genetic Analyzer and GeneMapper® software. Red arrows indicate alleles not present in the normal sample. Note that the four new LMR markers (BAT-52, BAT-56, BAT-59 and BAT-60) are somewhat more prone to heterozygosity, with multiple peaks in the normal sample. After comparing the tumor sample to its matched normal reference sample, microsatellite instability is noted at all markers and is considered MSI-high (MSI-H).

Representative Sample Demonstrating Shift Sizes by Markers

Allele shift size for MSI-high endometrial cancer tumor sample

MSI Marker shift comparison for long mononucleotide markers — Table showing average allele shift size for example MSI-high endometrial cancer tumor sample. Note that the shift in sizes of the alleles at the LMR markers are larger (average = 22.6bp) than the shifts detected in the traditional short markers (average = 7.0bp).

Long Markers Offer a Different Perspective on MSI

Tumor and normal endometrial cancer samples analyzed using the LMR MSI Analysis System — A matched pair of tumor and normal endometrial cancer samples were analyzed using the LMR MSI Analysis System. At the Gold Standard Marker, the unstable locus exhibited a small shift (denoted by the bracket) which can be difficult to classify. At the LMR Marker, the longer LMR locus exhibited a larger shift that is less ambiguous.

Seamless Support for Adopting LMR MSI in Your Research Lab

Our team is dedicated to partnering with you to ensure success and has broad experience training laboratories. We offer an online user training course as well as an in-person training option to get your lab up and running efficiently with the LMR MSI Analysis System.

Contact Us for Support

MSI Analysis is a Valuable Tool in Research and Tumor Screening

MSI as a Research Marker for Immunotherapeutic Response. Presented at the 2017 Annual Meeting of the Association of Molecular Pathology by Dr. James R. Eshleman.

Universal Tumor Screening for Lynch Syndrome: Past, Present and Future. Presented in 2018 by Heather Hampel, MS, LGC.

MSI panels and bins for GeneMapper® software can be downloaded for consistent data analysis. Panels and bins text files simplify and standardize data analysis by providing automated assignment of genotypes using GeneMapper® 4.0, 4.1, 5.0 and 6.0 software.

Download Panels and Bins

Protocols

Complete Protocol

LMR MSI Analysis System Technical ManualPDF (4364 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
LMR MSI 5X Primer Mix	MD232A	1 × 200μl
LMR MSI 5X Master Mix	MD233A	1 × 200μl
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
WEN Internal Lane Standard 500	DG500A	1 × 200μl		View Product
Water, Amplification Grade	DW099A	1 × 1,250μl		View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

TMR-ET, CXR-ET and WEN dyes are proprietary.

Resources

Articles

Citations

Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer.
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2016 Nat. Commun.
See all citations

Other Resources

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LMR MSI Analysis System

Characterize MSI Status of Challenging Research Samples

Resolve Microsatellite Instability Status in Challenging Sample Types

Increase MSI Detection Ability with Long Mononucleotide Repeat Markers

Validation of LMR Markers for MSI Detection