Maxwell® CSC Genomic DNA Kit

High-Efficiency, Automated Genomic DNA Extraction from a Broad Range of Sample Types

The Maxwell® CSC Genomic DNA Kit is intended for use as an in vitro diagnostic (IVD) medical device. The kit is used in combination with the Maxwell® CSC or CSC 48 Instrument and the Maxwell® CSC Genomic DNA method to extract and purify genomic DNA from multiple different human sample types in a single instrument run. Compatible sample types include human whole blood, buffy coat, bone marrow, buccal swabs, tissues, and cells isolated from tissue cultures or various biological fluids (e.g., urine, amniotic fluid, etc.). The Maxwell® CSC Genomic DNA Kit offers clinical laboratories a platform solution with built-in flexibility for consistent and reliable DNA extraction from different sample types and input amounts for use with a variety of in vitro diagnostic workflows such as PCR and qPCR assays.

Product is currently only available in Austria, Belgium, Canada, Croatia, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Korea, Latvia, Luxembourg, Netherlands, Norway, Poland, Portugal, Romania, Saudi Arabia, Singapore, Slovakia, Slovenia, Spain, Sweden, Switzerland, United Kingdom and United States.

Merche Bermejo Villodre, Laboratory Manager – Valenciahealthincode

The kit gives us a very good yield and quality of DNA from different kinds of samples compared to other kits that we have used. The most interesting feature is that we get good results even from samples that are originally expected to have low amounts of DNA and we are able to use one kit, for all samples at the same time.

Merche Bermejo Villodre, Laboratory Manager – Health In Code, Valencia

Flexible Workflow Allows Multiple Sample Types to be Processed in a Single Extraction Run

The Maxwell® CSC Genomic DNA Kit contains all the necessary reagents for DNA extraction from different sample types. All sample types use the same Maxwell® CSC Instrument method (~40 minutes) following a sample-specific lysis step. The Maxwell® CSC Genomic DNA Kit allows different sample input amounts, and the purified DNA can be eluted in 50–200µl of Elution Buffer.

Sample Types Compatible with the Maxwell® CSC Genomic DNA Kit

Maxwell CSC Genomic DNA Kit purifies DNA from whole blood, buffy coat or bone marrow samples

Maxwell CSC Genomic DNA Kit purifies DNA from buccal swabs, cells or tissue.

Simultaneous DNA Extraction on Maxwell® CSC Instruments in IVD Mode

Schematic of sample addition and sample mixing in the Maxwell cartridge.

Schematic of particle capture and nucleic acid binding steps in the Maxwell DNA purification method.

Schematic of washing and elution steps in the Maxwell DNA purification method.

Maxwell® CSC Genomic DNA Kit Extracts High-Quality DNA from Different Human Sample Types

Genomic DNA yields of whole blood with 4 different anticoagulants.

DNA extraction sample quality chart of absorbance-based purity ratios.

Concentration of DNA samples from Panel B.

The Maxwell® CSC Genomic DNA Kit used with the Maxwell® CSC or the Maxwell® CSC 48 Instrument consistently extracts high yield and quality of DNA from multiple sample types. Panel A. Genomic DNA was extracted from 300µl of whole blood (n=12) with different anticoagulants and eluted in either 50µl or 200µl of Elution Buffer. Panel B. Average purity ratios of DNA extracted from different sample types as determined by absorbance. Panel C. DNA was extracted from 300µl each of buffy coat and bone marrow, 5 × 10⁶ HEK293 cells, 50mg of pancreatic tissue, and one or two buccal swabs. All samples were eluted in 200µl of Elution Buffer, and the concentration and quality of samples was determined by absorbance.

Reliable Amplification using Genomic DNA Extracted from Different Human Sample Types using the Maxwell® CSC Genomic DNA Kit

DNA extracted using the Maxwell® CSC Genomic DNA Kit shows consistent amplification in qPCR.

DNA extracted from multiple sample types using the Maxwell® CSC Genomic DNA Kit shows consistent amplification in a qPCR assay. DNA was extracted from whole blood, buffy coat, bone marrow, HEK293 cells, pancreatic tissue and one or two buccal swabs (n=12) using the Maxwell® CSC Genomic DNA Kit on either the Maxwell® CSC or Maxwell® CSC 48 Instrument. The DNA was quantified by absorbance, diluted to a concentration within the qPCR standard curve and used as a template to amplify a 300-base-pair target in a qPCR assay.

Protocols

Complete Protocol

Maxwell® CSC Genomic DNA Kit Technical ManualPDF (2649 KB) – English

Video Protocols

What's in the box?

Item	Part #	Size	Available Separately
Maxwell® CSC Cartridge (CSCQ)	AS132B	48 × 1 each
CSC/RSC Plungers	AS133A	1 × 50/pk	View Product
Elution Tubes (0.5ml)	AS620A	1 × 50/pk	View Product
Lysis Buffer	MC110A	1 × 20ml
Proteinase K (PK) Solution	MC111A	2 × 1ml
Elution Buffer	MC112A	1 × 20ml
RNase A Solution	MC120A	1 × 1ml
Lytic Enhancer (LE2)	MC153A	1 × 20ml

SDS

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Certificate of Analysis

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Use Restrictions

For In Vitro Diagnostic Use. This product is only available in certain countries.

Storage Conditions

Resources

Software and Firmware

Other Resources

Maxwell® CSC Instruments Brochure

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Frequently Used With

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