Maxwell® HT Fecal Microbiome DNA Kit

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Automated DNA Extraction from Feces

  • Simple, automated DNA extraction with minimal hands-on time
  • Efficient inhibitor removal enables qPCR, 16S rRNA gene sequencing and shotgun metagenomic sequencing downstream
  • Rapid bead-beating-free protocol; efficient bead-beating protocols for demanding applications

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Catalog number selected: A6040

$ 1,575.00
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Maxwell® HT Fecal Microbiome DNA Kit
4 × 96 preps
$ 1,575.00
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Ready-to-Go DNA Extraction Kit, Expert Support Available 

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The Maxwell® HT Fecal Microbiome DNA Kit is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.

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When you purchase a Promega extraction solution, you gain free access to our elite Field Support Scientists, method development services, and technical support. Contact us if you need help setting up the Maxwell® HT Fecal Microbiome DNA Kit on your robot.

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With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.

Automated Microbiome DNA Extraction at Scale

  • High Yield
  • High Concentration
  • High Throughput

The Maxwell® HT Fecal Microbiome DNA Kit brings the same reagent chemistry used in the benchtop Maxwell® RSC instrument and makes it available at scale for use on high-throughput robotic systems.

The unique inhibitor removal chemistry used in the Maxwell® Systems enables simple automated hands-free DNA purification.

NGS-Ready Microbiome DNA Extraction

The example data below show the results of 16S V3/V4 metagenomic sequencing analysis for a microbial community standard and fecal samples purified with the Maxwell® RSC Fecal Microbiome DNA Kit with optional bead-beating. Experimental details are provided in the Application Note, Automated Extraction of Fecal DNA with a Bead-Beating Step. 
Sequencing results from DNA purified with Maxwell chemistry.
Sequencing results for the species-level bacterial profile of DNA purified from the microbial community standard as compared to the theoretical composition accounting for 16S copy number.
Sequencing results from DNA purified from stool samples using Maxwell chemistry.
Sequencing results for the genus-level bacterial profile of DNA purified from triplicate stool samples from two individuals. Sequencing data was analyzed using an internal analysis pipeline running the Mothur2 open-source software package. 

Broad Utility: Automated DNA Extraction from Feces and Other Sample Types

The Application Notes listed below contain protocols and example data using the Maxwell® HT Fecal Microbiome Kit chemistry to purify DNA from various sample types.

DNA Extraction Protocols image-56882596

Microbiome DNA Extraction Resources

Webinar Series

Register for one of our on-demand webinars to learn more about trends in microbiome research.

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Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Maxwell® HT C Resin

 A7751 4 × 5.5ml View Product

Wash Buffer (WBA)

 A176C 2 × 350ml View Product

25mM Tris-HCl (pH 8.0)

 A2641 1 × 60ml View Product

Proteinase K (PK) Solution

 A5051 1 × 23ml View Product

RNase A Solution

 A797E 2 × 5ml 4mg/ml View Product

Lysis Buffer

 MC141A 2 × 100ml View Product

Binding Buffer

 MC501E 1 × 160ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

Other Resources

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