Maxwell® RSC PureFood GMO and Authentication Kit

Simple, Highly Reproducible DNA Extraction from Food and Feed Samples

The Maxwell® RSC PureFood GMO and Authentication Kit provides an easy and automated method for efficient purification of DNA used in PCR-based testing for Genetically Modified Organism (GMO) DNA sequences and PCR based food and ingredient authentication. The Kit can purify DNA from raw and processed food samples including corn, soybeans, canola, ground pork and ground beef.

The Kit is used with the Maxwell® RSC and RSC 48 Instruments, which are supplied with preprogrammed purification methods and use predispensed reagent cartridges to maximize simplicity and convenience. The Maxwell® RSC Instrument can process from 1 to 16 samples and the Maxwell® RSC 48 can process from 1 to 48 samples in a single run.

Easy Purification Workflow

Purify your DNA in five easy steps. The protocol requires only 30 minutes hands-on time. It has fewer processing steps than the competitor and doesn't require any chloroform.

Add CTAB, RNase, and Proteinase K.
Vortex.
Incubate for 30 minutes (difficult samples can be incubated for an additional 2 hours).
Centrifuge for 10 minutes.
Add 300μl of lysate and 300μl of Lysis Buffer into well 1 of the Maxwell® RSC cartridge—run time is 40 minutes.

Load the Maxwell® deck, add sample, and press start. It's as simple as that.

~100 minute protocol

Only 30 minutes hands-on time
Fewer processing steps
No chloroform

Add lysis buffer and proteinase K.
Vortex.
Incubate for 30 minutes at 60°C.
Centrifuge for 5 minutes.
Add chloroform to empty tubes.
Transfer supernatant from centrifuge step 4 and add to chloroform tubes.
Vortex.
Centrifuge for 15 minutes.
Add buffer 1 to a clean tube, add aqueous phase from step 8 and vortex.
Add solution to spin column.
Centrifuge for 1 minute.
Discard flow through and add buffer 2.
Centrifuge, discard flow through and centrifuge again.
Add buffer 3.
Incubate for 1 minute.
Centrifuge for 1 minute.

~120 minute protocol

90 minutes hands-on time
More processing steps
Requires chloroform

Consistently Purify DNA from Raw and Processed Food Samples

The PureFood GMO and Authentication Kit can be used successfully with either raw or processed food samples. Below we present data for DNA extractions from raw ground pork, raw ground beef, chocolate and rice cakes.

Concentration of DNA obtained from raw beef and pork.

Concentration of DNA obtained from processed food.

DNA Extracted with the PureFood Kit Is Ready for Downstream Analysis

DNA extracted using the PureFood Kit can be used directly in a variety of downstream applications such as real-time PCR, gel electrophoresis, microarrays, and sequencing (e.g., NGS and Sanger).

13761MA-W — DNA was extracted from 100mg samples of ground soy, corn and canola. DNA from cod, pork and beef was extracted from 40mg of raw ground samples. Purity was determined by spectroscopy. Good absorption ratios falling above 1.8 for A_260/A₂₈₀ and between 1.8 and 2.4 for A₂₆₀/A₂₃₀.

Advantages

Use an optimized system to extract DNA from many food samples.
Experience less variability compared to traditional and competing extraction and purification methods.
Achieve faster results with walk-away automation and free up laboratory resources for higher value activities.
Avoid organic extractions and perform minimal hands-on steps to quickly purify DNA from up to 16 samples in less than 60 minutes.

Applications

DNA extracted using the PureFood Kit is ready for:

real-time PCR
gel electrophoresis
microarrays
Next-Generation and Sanger Sequencing

Protocols

Complete Protocol

Maxwell® RSC PureFood GMO and Authentication KitPDF (808 KB) – English

Quick Protocols

Maxwell® RSC PureFood GMO and Authentication Kit for Food, Feed and Seed Samples Quick Protocol FB201PDF (504 KB) – English

Maxwell® RSC PureFood GMO and Authentication Kit for Meat Samples Quick Protocol FB202PDF (502 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
RNase A Solution	A797C	2 × 1ml	4mg/ml	View Product
Elution Buffer	A828D	1 × 20ml		View Product
Maxwell® RSC Cartridge (RSCJ)	AS159A	48 × 1 each
Elution Tubes (0.5ml)	AS620A	1 × 50/pk		View Product
CTAB Buffer	MC1411	1 × 100ml		View Product
Proteinase K (PK) Solution	MC500C	2 × 1ml		View Product
Lysis Buffer	MC501C	1 × 20ml
RSC Plungers	AS1670	1 × 48/pack		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 6,855,499, European Pat. No. 1368629, Japanese Pat. No. 4399164 and other patents pending.

Resources

Articles

Modified Protocol for Extracting RNA-free Plant DNA

Software and Firmware

Posters

Poster: Identifying Food Adulteration: Reliable and Sensitive Detection of Contaminants in Halal Foods, Camel Milk, and Coffee Beans

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Maxwell® RSC PureFood GMO and Authentication Kit

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