ReliaPrep™ FFPE gDNA Miniprep System

ReliaPrep FFPE gDNA Miniprep System 100 reactions
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Intact, Amplifiable DNA from FFPE Tissue

  • Minimal preparation time; simply add ethanol and go!
  • Safely deparaffinize without using harsh organic solvents
  • No overnight digestion required

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Catalog number selected: A2351

$ 123.00
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

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ReliaPrep™ FFPE gDNA Miniprep System
10 reactions
$ 123.00
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Higher Quality DNA from FFPE Samples

ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.

The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.

The protocol is complete in 2.5 hours with minimal hands-on time.

ReliaPrep™ FFPE Miniprep Systems Help Preserve DNA Integrity

10142TB-BLKtype

In this experiment, 10µl of gDNA purified from FFPE human liver samples was used as template in multiplex PCR to amplify fragments of the GAPDH gene. DNA fragments ranging from 100–400bp were detected.

Purified FFPE DNA is Compatible with Sensitive Assays

Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641).

10187ta

Simple Protocol

10146ME-W

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: A2351 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Collection Tubes

 A130C 1 × 10/pk

Mineral Oil

 A244A 1 × 5ml

ReliaPrep™ FFPE Binding Columns

 A247A 1 × 10/pk

Proteinase K (PK) Solution

 A505A 1 × 250μl View Product

RNase A Solution

 A797D 1 × 200μl 4mg/ml View Product

Elution Buffer

 A828C 1 × 15ml View Product

Lysis Buffer (LBA)

 Z101A 2 × 1ml

BL Buffer

 Z103A 1 × 3.25ml

Wash Solution

 Z104A 1 × 3ml

Blue Dye

 MC122A 1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2352 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Collection Tubes

 A130A 2 × 50/pk

Mineral Oil

 A244B 1 × 50ml

ReliaPrep™ FFPE Binding Columns

 A247B 2 × 50/pk

Proteinase K (PK) Solution

 A505B 2 × 1.1ml View Product

RNase A Solution

 A797C 1 × 1ml 4mg/ml View Product

Elution Buffer

 A828C 1 × 15ml View Product

Lysis Buffer (LBA)

 Z101B 1 × 30ml

BL Buffer

 Z103B 1 × 32.5ml

Wash Solution

 Z104B 1 × 30ml

Blue Dye

 MC122A 1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

Citations

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