Wizard® SV Genomic DNA Purification System

Simple DNA Purification Method for Cells and Tissues

The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 10⁶ cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.

The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).

Sample Types Processed With Wizard® SV Genomic

Blood

Sample Type	Promega	External	Comments
Peripheral blood mononuclear cells		Yes	Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705. PubMed ID #16361556.
Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes		Yes	Morii, E. et al. (2004) Blood 104, 1656–61. PubMed ID #15172970.

Mammalian Tissues

Sample Type	Promega	External	Comments
Mouse liver	Yes		A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse brain	Yes		A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse heart	Yes		A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse spleen		Yes	Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42. PubMed ID #16498502.
Mouse ear		Yes	Du, Q. et al. (2006) FASEB J. 20, 1131–41. PubMed ID #16770012.
Mouse skin		Yes	Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation. Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14. PubMed ID #16818633.
Anopheles funestus mosquito carcass		Yes	Michel, A.P. et al. (2006) Genetics 173, 1389–95. PubMed ID #16648581.
Human lung cancer biopsy		Yes	Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9. PubMed ID #15837743.
Human distant normal colorectal mucosa and tumor tissue		Yes	Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7. PubMed ID #15485994.
Human frozen thymic epithelial tumor samples		Yes	Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6. PubMed ID #16762968.
Human pancreatic islet		Yes	Del Guerra, S. et al. (2005) Diabetes 54, 727–35. PubMed ID #15734849.

Cultured Cells

Sample Type	External	Comments
Chicken retinospheroid cultures	Yes	Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24. PubMed ID #16207942.
HT1080 human fibrosarcoma	Yes	Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction. Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80. PubMed ID #16973543.
Monkey primary fibroblasts, virus-infected	Yes	Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated. Blanchard, E. et al. (2006) J. Virol. 80, 6964–72. PubMed ID #16809302.

Bacteria

Sample Type	External	Comments
E. coli K-12 derivative, MC4100	Yes	Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21. PubMed ID #13129953.
Pseudomonas aeruginosa	Yes	Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903. PubMed ID #15161855.
Bacillus subtilis	Yes	Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3. PubMed ID #14973012.
Eleven Gram-negative bacterial species	Yes	Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C. Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488.
Eleven Gram-positive bacterial species	Yes	Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C. Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488.

Other Organisms and Tissues

Sample Type	Promega	External	Comments
Snail cercariae		Yes	Smith, P. et al. (2004) J. Immunol. 173, 1240–8. PubMed ID #15240716.

Protocols

Complete Protocol

Wizard® SV Genomic DNA Purification System Technical BulletinPDF (620 KB) – English

Quick Protocols

Wizard SV Genomic DNA Purification System Quick Protocol FB069PDF (249 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
Wizard® SV Minicolumns	A129A	1 × 50/pk
Collection Tubes	A130A	1 × 50/pk
Column Wash Solution (CWA)	A1311	1 × 185ml	View Product
Nuclei Lysis Solution	A7941	1 × 50ml	View Product
RNase A Solution	A797A	1 × 250μl	View Product
Nuclease-Free Water	P119C	2 × 25ml	View Product
EDTA, 0.5M (pH 8.0)	V423B	1 × 30ml	View Product
Wizard® SV Lysis Buffer	Z3052	1 × 50ml	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Wizard® SV Minicolumns	A129B	1 × 250/pk
Collection Tubes	A130B	1 × 250/pk
Column Wash Solution (CWA)	A1311	2 × 185ml		View Product
Nuclei Lysis Solution	A7941	1 × 50ml		View Product
RNase A Solution	A797C	2 × 1ml	4mg/ml	View Product
Nuclease-Free Water	P1195	1 × 150ml		View Product
EDTA, 0.5M (pH 8.0)	V423B	1 × 30ml		View Product
Wizard® SV Lysis Buffer	Z3052	2 × 50ml		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Citations

Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer.
--
2016 Nat. Commun.
See all citations

Other Resources

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Wizard® SV Genomic DNA Purification System

Extracts DNA from Tissues or Cells

Simple DNA Purification Method for Cells and Tissues

Sample Types Processed With Wizard® SV Genomic

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Citations

Other Resources

Vac-Man® Jr. Laboratory Vacuum Manifold

Vac-Man® Laboratory Vacuum Manifold

Proteinase K (Lyophilized)

QuantiFluor® ONE dsDNA System

Nuclei Lysis Solution

Wizard® SV Lysis Buffer

Column Wash Solution (CWA)

Wizard® SV Genomic DNA Purification System

Extracts DNA from Tissues or Cells

Simple DNA Purification Method for Cells and Tissues

Sample Types Processed With Wizard® SV Genomic

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Citations

Other Resources

Similar Products

Wizard® SV 96 Genomic DNA Purification System

ReliaPrep™ gDNA Tissue Miniprep System

Maxwell® RSC Tissue DNA Kit

Maxwell® RSC Cell DNA Purification Kit

Frequently Used With

Vac-Man® Jr. Laboratory Vacuum Manifold

Vac-Man® Laboratory Vacuum Manifold

Proteinase K (Lyophilized)

QuantiFluor® ONE dsDNA System

Nuclei Lysis Solution

Wizard® SV Lysis Buffer

Column Wash Solution (CWA)

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