Maxwell® RSC miRNA from Tissue or Plasma and Serum Kits

Purify High-Quality miRNA from Tissue, Plasma, Serum or Enriched Exosomes

The Maxwell^® RSC miRNA Tissue Kit purifies total RNA, including microRNA (miRNA), from 1–48 tissue samples in about 90 minutes. The Maxwell^® RSC miRNA Plasma and Serum Kit will purify total RNA, including miRNA, from 1–48 samples in about 70 minutes. The kits provide all the reagents required for sample processing and use prefilled cartridges for purification, simplifying extraction and freeing up your time for analysis.

The Maxwell^® RSC miRNA Tissue and miRNA Plasma and Serum Kits work with both the Maxwell® RSC and RSC 48 instruments. The Maxwell^® RSC Instrument can process from 1 to 16 samples and the Maxwell^® RSC 48 can process from 1 to 48 samples in a single run. The Maxwell® RSC miRNA Tissue and miRNA Plasma and Serum Kits are also compatible for use on the Maxwell® CSC Instrument in RUO Mode.

The extracted RNA is highly concentrated and ready for use in RT-qPCR, RNA sequencing and other downstream applications used in biomarker detection, gene expression and other studies.

No clogging worries with a paramagnetic particle mover

Because the Maxwell® RSC Instruments are magnetic particle movers, not liquid handlers, they offer advantages over other automated systems. There is minimal risk of cross-contamination because no liquid handling of splattering happens during sample processing. With no clogs and fewer breakdowns, there are fewer disruptions in your workflow.

Simple, safe and efficient protocol

Purify RNA that is ready for use in downstream assays with minimal preprocessing and no organic reagents.

Using tissue

Automated miRNA extraction from tissue protocol

Using plasma, serum or enriched exosomes

Automated miRNA extraction from plasma serum or exosomes protocol

Consistent purification of multiple miRNA targets

miRNA from tissue

Multiple miRNA targets can be consistently purified from mammalian tissues. The high-quality RNA is ready to use in downstream amplification assays such as RT-qPCR.

high yields of automated miRNA extraction from tissue

high yields of automated miRNA extraction from different tissues — **miRNA purified from pancreas, kidney and liver samples.** miRNA yield was quantitated for both mir-21 and let-7a targets by RT-qPCR using TaqMan®-based miRNA-specific primers and standards.

miRNA from plasma

Consistent purification of high-quality miRNA from plasma, serum or enriched exosomes.

high yields of automated miRNA extraction from plasma — **miRNA purified from plasma.** Comparison of the total yield of miR-21 purified from 200μl of plasma using the Maxwell® RSC miRNA Plasma and Serum Kit or the miRNeasy Serum/Plasma Kit (Qiagen). N = 4.

Protocols

Complete Protocol

Maxwell® RSC miRNA Tissue Kit Technical ManualPDF (1473 KB) – English

Maxwell® RSC miRNA Plasma and Serum Kit Technical ManualPDF (1437 KB) – English

Quick Protocols

Maxwell RSC miRNA Tissue Kit FB188PDF (421 KB) – English

Maxwell® RSC miRNA Plasma and Serum Kit Quick ProtocolPDF (217 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
1-Thioglycerol	A208B	1 × 900μl
Maxwell® RSC Cartridge (RSCN)	AS173A	48 × 1 each
Elution Tubes (0.5ml)	AS620A	1 × 50/pk	View Product
Blue Dye	C888A	1 × 50μl
Proteinase K (PK) Solution	MC500C	2 × 1ml	View Product
Lysis Buffer	MC501C	1 × 20ml
Nuclease-Free Water	P119C	1 × 25ml	View Product
Homogenization Solution	Z305H	1 × 30ml
DNase I (lyophilized)	Z358A	2 × 1 vial	View Product
RSC Plungers	AS1670	1 × 48/pack	View Product
Lytic Enhancer	MC145A	1 × 20ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
RSC Plungers	AS1670	1 × 48/pack	View Product
Maxwell® RSC Cartridge (RSCL)	AS168A	48 × 1 each
Elution Tubes (0.5ml)	AS620A	1 × 50/pk	View Product
Blue Dye	C888A	1 × 50μl
Lysis Buffer C	MC136A	1 × 25ml
Proteinase K (PK) Solution	MC500C	5 × 1ml	View Product
Nuclease-Free Water	P119C	1 × 25ml	View Product
DNase I (lyophilized)	Z358A	2 × 1 vial	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Software and Firmware

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Purify High-Quality miRNA from Tissue, Plasma, Serum or Enriched Exosomes

No clogging worries with a paramagnetic particle mover