ReliaPrep™ miRNA Cell and Tissue Miniprep System

Purify concentrated RNA quickly and safely

The ReliaPrep™ miRNA Cell and Tissue Miniprep System isolates total RNA including microRNA (miRNA) and other small non-coding RNA (sncRNA) subspecies from a variety of cell and tissue types. The system reliably and consistently purifies RNA from as few as 100 to as many as 1 × 10⁶ cultured cells, and from 0.25 to 20mg of tissue. Purified RNA is extracted in a low elution volume (as low as 15μl) ready for use in your downstream assays.

Organic-based purification has been accepted as the most sensitive method to purify RNA. The ReliaPrep™ miRNA purification system achieves virtually the same yield and purity of amplifiable miRNA without phenol:chloroform extraction, eliminating the need to handle and dispose of harsh organic products.

Purified, concentrated RNA includes miRNA and subspecies

RNA Species from Cells

13587MB-W — Expression of miRNA (miR16), snoRNA (U6), and sncRNA (RNU44 and RNU48) isolated from HEK293 cells.

RNA Species from Tissue

13588MC-W — Expression of miRNA and snoRNA products detected from total RNA isolated from 10mg of frozen mouse liver and kidney tissue.

RNA can be purified from small sample sizes

The ReliaPrep column/binding matrix efficiently captures RNA from very small amounts of input material.

RNA from 10–10⁵cells

13586MB-W-a — Here, the miRNA targets U6 and miR16 were amplified after RNA isolation from varying cell culture inputs. RNA was purified from as few as 10 cells to as many as 1x10⁵ HEK293 cells. miR16 and U6 expression levels were determined using qPCR.

RNA from 0.0032–10mg Tissue

13586MB-W-b — In this example, the miRNA targets let7e and miR16 were reliably amplified from ReliaPrep-purified RNA isolated from 0.0032–10mg frozen mouse kidney tissue. miR16 and let7e expression levels were determined by qPCR.

Isolate pure RNA without phenol:chloroform extraction

The ReliaPrep™ miRNA System does not use phenol chloroform yet results in acceptably pure RNA isolated from a range of tissue types. The data below show purity rations for RNA purified from various mouse tissues using the ReliaPrep™ miRNA Cell and Tissue Miniprep System and other commercial miRNA purification kits.

RNA purity A260/A280

13584MB-W-a — All kits purified RNA with A₂₆₀/A₂₈₀ purity ratios in the accepted 1.8–2.2 range.

RNA purity A260/A230 ratios

13584MB-W-b — Kits using phenol chloroform reagents generated RNA with A₂₆₀/A₂₃₀ ratios below the accepted 1.8–2.2 range.

Download the poster

MicroRNA Analysis Paired with a Novel Live Cell Viability Assay: A Complete Epigenetic Workflow in Human Cancer Cell Lines

In this poster, the authors used total RNA, mRNA and miRNA isolated with the Reliaprep™ miRNA system to examine the role of small or non-coding RNAs in cancer cell growth and metastasis.

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Protocols

Complete Protocol

ReliaPrep™ miRNA Cell and Tissue Miniprep SystemPDF (742 KB) – English

Quick Protocols

ReliaPrep miRNA Cell and Tissue Miniprep System FB205PDF (322 KB) – English

ReliaPrep miRNA Cell and Tissue Miniprep System FB206PDF (322 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
Collection Tubes	A130C	2 × 10/pk
ReliaPrep™ Minicolumns	A207A	2 × 10/pack
1-Thioglycerol	A208A	1 × 75μl
DNase Buffer, 10X	M198B	1 × 500μl
Nuclease-Free Water	P119A	1 × 1,250μl	View Product
Lysis Buffer (LBA)	Z106A	1 × 6ml
Elution Tubes	Z200B	1 × 25/pk
RNA Wash Solution (RWA)	Z309B	1 × 11.8ml	View Product
DNase I (lyophilized)	Z358B	1 × 1 vial	View Product
RNA Dilution Buffer (RDB)	Z372C	1 × 9ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Collection Tubes	A130A	2 × 50/pk
ReliaPrep™ Minicolumns	A207B	2 × 50/pack
1-Thioglycerol	A208B	1 × 900μl
DNase Buffer, 10X	M198B	1 × 500μl
Nuclease-Free Water	P119E	1 × 13ml	View Product
Lysis Buffer (LBA)	Z106B	1 × 30ml
Elution Tubes	Z200C	2 × 50/pk
RNA Wash Solution (RWA)	Z3091	1 × 58.8ml
DNase I (lyophilized)	Z358A	1 × 1 vial	View Product
RNA Dilution Buffer (RDB)	Z372A	1 × 44ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Collection Tubes	A130B	2 × 250/pk
ReliaPrep™ Minicolumns	A207B	10 × 50/pack
1-Thioglycerol	A208B	4 × 900μl
DNase Buffer, 10X	M198B	3 × 500μl
Nuclease-Free Water	P119C	1 × 25ml	View Product
Lysis Buffer (LBA)	Z106C	1 × 150ml
Elution Tubes	Z200C	10 × 50/pk
RNA Wash Solution (RWA)	Z309D	1 × 206ml	View Product
DNase I (lyophilized)	Z358A	5 × 1 vial	View Product
RNA Dilution Buffer (RDB)	Z372B	1 × 220ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Citations

Other Resources

MicroRNA Analysis Paired with a Novel Live Cell Viability Assay: A Complete Epigenetic Workflow in Human Cancer Cell Lines

Frequently Used With

RNasin® Ribonuclease Inhibitor

The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases.

N2111, N2115, N2511, N2515, N2611, N2615

RNA Wash Solution (RWA)

Component of several RNA purification systems available for separate purchase.

Z3091

ReliaPrep™ miRNA Cell and Tissue Miniprep System

Fast, Phenol-Free miRNA Isolation from Cells and Tissues

Purify concentrated RNA quickly and safely