Viral RNA/DNA Concentration and Extraction Kits for Wastewater

Direct Capture of SARS-CoV-2 RNA in 2 Hours

The Enviro Total Nucleic Acid Kits for Wastewater include reagents and consumables to concentrate and purify total nucleic acids (TNA) in one convenient kit. Our unique vacuum-based direct capture method simplifies sample processing by concentrating and capturing viral TNA quicker than precipitation methods without the use of cumbersome ultracentrifugation, while achieving high and consistent yield. The entire process from sample to purified TNA takes less than 2 hours and is scalable and adaptable to your needs. The process also removes most PCR inhibitors, so the resulting TNA can be used directly for amplification of SARS-CoV-2 RNA using the SARS-CoV-2 RT-qPCR Kit for Wastewater.

We offer three kit formats based on sample throughput. Direct capture of nucleic acids is followed by purification using mini spin columns or benchtop automation using Maxwell® Instruments. A plate-based high-throughput kit is also available. An optional step can be included to extract nucleic acids from solid samples.

Choose A Format For Your Sample Throughput

Manual: Vacuum + Spin Column

The Wizard® Enviro Total Nucleic Acid Kit (A2991) allows you to get purified TNA in 90 minutes or less with a simple protocol. First, the TNA is directly captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is further cleaned using PureYield™ Minicolumns. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA.

Maxwell® Automation: Vacuum + Maxwell® Extraction

The Maxwell® RSC Enviro Total Nucleic Acid Kit (AS1831) allows you to get purified TNA in 2 hours with vacuum-based direct capture of TNA followed by automated TNA extraction using Maxwell® Instruments. The Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples in a single run. Viral TNA is first captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is loaded into the Maxwell® RSC Instrument for walkaway RNA extraction. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA.

High-Throughput Automation: Plate-Based

The Maxwell® HT Environmental TNA Kit (AX9190) can be used for high-throughput, automated extraction of viral TNA from wastewater. After an initial concentration with another method, samples are extracted in 96-well plates using magnetic separation. The kit can be used with any automated high-throughput platform. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.

High-Throughput Concentration and Extraction: Plate-Based

The Maxwell® HT Enviro Concentration and TNA Extraction Kit (CS344301) provides magnetic particle-based concentration of virus with Ceres Nanotrap® particles from 10ml of wastewater followed by extraction of TNA from the concentrate. The reagents are formatted for use with laboratory automation for walkaway sample processing with minimal user intervention. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.

Simple Direct Capture Protocol

The filter-based Direct Capture method allows rapid purification and concentration of viral nucleic acids from large volumes of wastewater samples.

Overview of direct capture method for rapid purification and concentration of viral nucleic acid from wastewater

Direct Capture Results in Higher Yield Than PEG/NaCl

wastewater concentration SARS-CoV-2 RNA yield comparison — Preliminary data shows the direct capture method resulted in 30 times higher SARS-CoV-2 RNA yield compared to PEG/NaCl. Wastewater samples were collected from a major US city.

Select Which Product is Right for You

Learn more about wastewater surveillance methods and tools.

Cat.#	Name	Concentration Method	Input Sample Volume	Intended Throughput	Size	Format
A2991	Wizard® Enviro TNA Kit	Vacuum-Based	40ml	<16	25 preps	Manual Spin Column
AS1831	Maxwell® RSC Enviro TNA Kit	Vacuum-Based	40ml	1–48	48 preps	Semi-Automated
CS347901	Maxwell® RSC Enviro Nanotrap® A TNA Kit	Ceres Nanotrap® Microbiome A particles	10ml	>48	48 preps	Semi-Automated
CS344301	Maxwell® HT Enviro Nanotrap® A TNA Kit	Ceres Nanotrap® Microbiome A particles	10ml	>48	4 × 96 preps	High-Throughput
AX9190	Maxwell® HT Enviro Kit	Not Included	1ml	>48	4 × 96 preps	High-Throughput

What Customers Are Saying

“We are very happy to have implemented the large volume extraction kits in our analysis of wastewater for SARS-CoV-2. The integration of concentration and extraction steps has been instrumental in expediting our workflow. The kits also provide higher reproducibility between biological replicates compared to the method we were using previously.”

Jeseth Delgado Vela
Asst. Professor, Howard University

“We have tested the new Promega Wastewater Kit and were very pleased with results. In my side-by side comparison, the Promega Wastewater Kit has demonstrated way higher viral RNA recovery. We are planning to use it for all future wastewater work.”

Sergey Balashov
PCR Laboratory Director, EMSL Analytical

Have questions? We're here to help!

Contact Us

Protocols

Complete Protocol

Wizard® Enviro Total Nucleic Acid Kit Technical Manual PDF (2205 KB) – English

Maxwell® RSC Enviro Total Nucleic Acid Kit Technical ManualPDF (2300 KB) – English

Quick Protocols

Wizard Enviro Total Nucleic Acid Kit FB236PDF (927 KB) – English

Maxwell RSC Enviro Total Nucleic Acid Kit FB237PDF (966 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
Binding Buffer 1 (BBD)	A2981	1 × 320ml	View Product
Binding Buffer 2 (BBE)	MC1501	1 × 30ml	View Product
Protease Solution	A144C	1 × 13ml	View Product
Column Wash 1 (CWE)	A296A	1 × 85.3ml
Column Wash 2 (RWA)	A297A	1 × 206ml
Nuclease-Free Water	P1195	1 × 150ml	View Product
PureYield™ Binding Columns	A245A	5 × 5 each
PureYield™ Minicolumns	A247C	1 × 25/pk
PureYield™ Collection Tubes	A167C	1 × 25/pk
Elution Tubes	Z200C	1 × 50/pk
Reservoir Extension Funnel	A3041	1 × 25/pk	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Column Wash 1 (CWE)	A296A	2 × 85.3ml
Column Wash 2 (RWA)	A297A	2 × 206ml
Nuclease-Free Water	P1195	1 × 150ml	View Product
PureYield™ Binding Columns	A245C	12 × 4 each
Elution Tubes (0.5ml)	AS620A	1 × 50/pk	View Product
Maxwell® RSC Cartridge (RSCJ)	AS159A	48 × 1 each
RSC Plungers	AS1670	1 × 48/pack	View Product
Elution Tubes	Z200C	1 × 50/pk
Binding Buffer 1 (BBD)	A2981	2 × 320ml	View Product
Binding Buffer 2 (BBE)	MC1501	2 × 30ml	View Product
Protease Solution	A1442	1 × 30ml	View Product
Reservoir Extension Funnel	A3041	2 × 25/pk	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Protease Solution, Custom	AX918A	1 × 60ml
Cell Lysis Buffer (CLD)	A173C	1 × 160ml	View Product
Resin	A175B	4 × 5.5ml	View Product
Wash Buffer (WBA)	A176C	3 × 350ml	View Product
CTAB Buffer	MC1411	1 × 100ml	View Product
25mM Tris-HCl (pH 8.0)	A2641	2 × 60ml	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Wizard® Enviro TNA Kit	A2991	1 × 25 preps
Welch Vacuum Pump for North America Electrical	A6720	1 × 1 each
Eluator™ Vacuum Elution Device	A1071	1 × 4 each	View Product
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity	A7231	1 × 1 each	View Product
Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper	A3110	1 × 1 each	View Product
Polypropylene Vacuum Flask, 1,700ml	AS1839	1 × 1 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Available Separately
Maxwell® RSC Enviro TNA Kit	AS1831	1 × 48 preps
Welch Vacuum Pump for North America Electrical	A6720	1 × 1 each
Eluator™ Vacuum Elution Device	A1071	1 × 4 each	View Product
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity	A7231	1 × 1 each	View Product
Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper	A3110	1 × 1 each	View Product
Polypropylene Vacuum Flask, 1,700ml	AS1839	1 × 1 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Viral Concentration and Viral RNA Purification from Wastewater on the KingFisher™ Flex Purification System

Software and Firmware

Other Resources

Similar Products

Pathogen Detection Kits for Wastewater

Detect pathogen RNA in preprocessed wastewater with high efficiency and sensitivity.

AM2100, AM2110, AM2120, AM2130, AM2140, AM2070, AM2050, AM2060, AM2150, AM2160

GoTaq® Enviro qPCR and RT-qPCR Systems

Ready-to-use master mixes optimized for amplifying targets from environmental samples.

AM2000, AM2001, AM2010, AM2011, AM2030, AM2040

Viral RNA/DNA Concentration and Extraction Kits for Wastewater

Direct Capture and Purification of Viral DNA/RNA From Wastewater

Direct Capture of SARS-CoV-2 RNA in 2 Hours

Choose A Format For Your Sample Throughput

Manual: Vacuum + Spin Column

Maxwell® Automation: Vacuum + Maxwell® Extraction

High-Throughput Automation: Plate-Based

High-Throughput Concentration and Extraction: Plate-Based

Simple Direct Capture Protocol

Direct Capture Results in Higher Yield Than PEG/NaCl

Select Which Product is Right for You

What Customers Are Saying

Have questions? We're here to help!

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Articles

Software and Firmware

Other Resources

Similar Products

Pathogen Detection Kits for Wastewater

GoTaq® Enviro qPCR and RT-qPCR Systems

Frequently Used With

Maxwell® RSC Instrument

Maxwell® RSC 48 Instrument

Vac-Man® Jr. Laboratory Vacuum Manifold

Eluator™ Vacuum Elution Device

Vac-Man® Laboratory Vacuum Manifold

Binding Buffer 1 (BBD)

Binding Buffer 2 (BBE)

Protease Solution

Americas

Pacific Asia

Europe