ReliaPrep™ FFPE gDNA Miniprep System

ReliaPrep FFPE gDNA Miniprep System 100 reactions
customize-this-small
View information on global supply logistics

Intact, Amplifiable DNA from FFPE Tissue

  • Minimal preparation time; simply add ethanol and go!
  • Safely deparaffinize without using harsh organic solvents
  • No overnight digestion required

Size

Catalog number selected: A2351

$ 123.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
ReliaPrep™ FFPE gDNA Miniprep System
10 reactions
$ 123.00
Your price: Log in
Change Configuration

Higher Quality DNA from FFPE Samples

ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.

The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.

The protocol is complete in 2.5 hours with minimal hands-on time.

ReliaPrep™ FFPE Miniprep Systems Help Preserve DNA Integrity

10142TB-BLKtype

In this experiment, 10µl of gDNA purified from FFPE human liver samples was used as template in multiplex PCR to amplify fragments of the GAPDH gene. DNA fragments ranging from 100–400bp were detected.

Purified FFPE DNA is Compatible with Sensitive Assays

Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641).

10187ta

Simple Protocol

10146ME-W

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: A2351 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Collection Tubes

 A130C 1 × 10/pk

Mineral Oil

 A244A 1 × 5ml

ReliaPrep™ FFPE Binding Columns

 A247A 1 × 10/pk

Proteinase K (PK) Solution

 A505A 1 × 250μl View Product

RNase A Solution

 A797D 1 × 200μl 4mg/ml View Product

Elution Buffer

 A828C 1 × 15ml View Product

Lysis Buffer (LBA)

 Z101A 2 × 1ml

BL Buffer

 Z103A 1 × 3.25ml

Wash Solution

 Z104A 1 × 3ml

Blue Dye

 MC122A 1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2352 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Collection Tubes

 A130A 2 × 50/pk

Mineral Oil

 A244B 1 × 50ml

ReliaPrep™ FFPE Binding Columns

 A247B 2 × 50/pk

Proteinase K (PK) Solution

 A505B 2 × 1.1ml View Product

RNase A Solution

 A797C 1 × 1ml 4mg/ml View Product

Elution Buffer

 A828C 1 × 15ml View Product

Lysis Buffer (LBA)

 Z101B 1 × 30ml

BL Buffer

 Z103B 1 × 32.5ml

Wash Solution

 Z104B 1 × 30ml

Blue Dye

 MC122A 1 × 100μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

Citations

Similar Products

Maxwell® RSC Xcelerate DNA FFPE Kit

An expedited process (<1 hour) for purifying DNA from FFPE tissue samples.

AS1510

Maxwell® RSC DNA FFPE Kit

Extracts DNA from FFPE tissue samples using the Maxwell® RSC Instrument.

AS1450, ASB1450

ReliaPrep™ FFPE Total RNA Miniprep System

Amplifiable total RNA from FFPE tissues without organic solvents or overnight digestion.

Z1001, Z1002

ReliaPrep™ Blood gDNA Miniprep System

Fast, manual purification of high-quality DNA from up to 200μl blood or body fluids.

A5081, A5082

Maxwell® HT DNA FFPE Isolation System

High-throughput purification of DNA from FFPE samples.

A6372

Frequently Used With

Molecular Biology Lab Guide

A resource designed for scientists just embarking on their career, focusing on fundamental technologies and techniques.

GEN1978

Diamond™ Nucleic Acid Dye

Sensitive fluorescent dye that binds to ssDNA, dsDNA and RNA. Stain and visualize nucleic acids in gels.

H1181

BenchTop 100bp DNA Ladder

Eleven fragments of 100–1,000bp in 100bp increments premixed with loading dye.

G8291

Wizard® SV Gel and PCR Clean-Up System

Purifies DNA fragments from gel slices or PCR in 15 minutes.

A9281, A9282, A9285, A9283, A9284

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.