GoTaq® Probe qPCR and RT-qPCR Systems

Simple, Sensitive Probe-Based qPCR and RT-qPCR

The GoTaq^® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These real-time qPCR systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.

Rapid hot-start activation and processive enzymes make the GoTaq^® Probe qPCR and RT-qPCR Systems compatible with most real-time PCR instruments using standard or fast cycling. Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.

Choose qPCR or RT-qPCR Master Mix Formats

Sensitivity and Specificity for qPCR

The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe qPCR Master Mix offers:

Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
Optimized formulation compatible with running multiplex assays.
Room-temperature setup, making it suitable for automation and high-throughput detection.
Resistance to a wide range of PCR inhibitors.

View GoTaq® Probe qPCR Master Mix Citations

Size	Cat.#	10µl Reactions	20µl Reactions
A6101	2ml	400 reactions	200 reactions
A6102	10ml	2,000 reactions	1,000 reactions

qPCR Probe kit example Cq values — **qPCR amplification from FFPE samples with GoTaq® Probe Master Mix**. DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Reverse Transcription and qPCR in One Reaction

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

View GoTaq® Probe 1-Step System Citations

Size	Cat.#	10µl Reactions	20µl Reactions
A6120	2ml	400 reactions	200 reactions
A6121	12.5ml	2,500 reactions	1,250 reactions

Real-Time RT qPCR Reagent Kit Data 13242MA — **Expression levels for the Zmm19 gene in corn.** Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).

Two-Step Reverse Transcription and qPCR

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

View GoTaq® 2-Step System Citations

Size	Cat.#	10µl Reactions	20µl Reactions
A6110	2ml	50 × 20µl RT reactions and 400 × 10µl qPCR reactions	50 × 20µl RT reactions and 200 × 20µl qPCR reactions

Real-Time qPCR Probe kit comparison data — **Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.**

Performance Tested in qPCR

We have generated a large collection of data using different instruments, templates, and sample types. Visit the qPCR Data Selector to see the results generated using the GoTaq® qPCR systems.

View qPCR Data

Suitable for Different Organisms and Sample Types

DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Organism Types:

Animal
Bacteria
Fungi
Human
Parasites
Viruses
Yeast

Sample Types:

Blood
Cells
Feces
FFPE
Food
Human Cerebrospinal Fluid (CSF)
Plants
Plasma
Saliva
Serum
Sputum
Swabs
Tissue
Urine

Compatible with these Instruments

For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
Applied Biosystems 7300 and 7900HT Real-Time PCR System
Applied Biosystems GeneAmp® 5700 Thermal Cycler
Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
Applied Biosystems ViiA® 7 Real-Time PCR System
Applied Biosystems QuantStudio® Real Time PCR Systems
Bio-Rad CFX96 Real-Time PCR Detection System
Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
Bio-Rad/MJ Research Chromo4™ Real-Time Detector
Bio-Rad MyiQ™ Real-Time PCR Detection System
Cepheid SmartCycler® System
Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
Eppendorf Mastercycler® ep realplex Real-Time PCR System
Roche LightCycler® 480 Real-Time PCR System
Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
Stratagene Mx4000® Multiplex Quantitative PCR System

Protocols

Complete Protocol

GoTaq® Probe qPCR Master Mix Technical ManualPDF (1196 KB) – English

GoTaq® Probe 1-Step RT-qPCR System Technical ManualPDF (1161 KB) – English

GoTaq® Probe 2-Step RT-qPCR System Technical ManualPDF (552 KB) – English

Quick Protocols

GoTaq Probe qPCR Master Mix Quick Protocol FB138PDF (330 KB) – English

GoTaq Probe 1 Step RT qPCR System Quick Protocol FB139PDF (260 KB) – English

GoTaq Probe 2 Step RT qPCR System Quick Protocol FB140PDF (250 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
GoTaq® Probe qPCR Master Mix, 2X	A610A	2 × 1ml
CXR Reference Dye	C541A	1 × 100μl	View Product
Nuclease-Free Water	P119A	2 × 1,250μl	View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GoTaq® Probe qPCR Master Mix, 2X	A610A	10 × 1ml
CXR Reference Dye	C541B	2 × 200μl	30μM	View Product
Nuclease-Free Water	P119E	1 × 13ml		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GoTaq® Probe Master Mix with dUTP	A612A	2 × 1ml
CXR Reference Dye	C541B	1 × 200μl	30μM	View Product
GoScript™ RT Mix for 1-Step RT-qPCR	M700A	1 × 225μl	50X
Nuclease-Free Water	P119A	2 × 1,250μl		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GoTaq® Probe Master Mix with dUTP	A612B	1 × 12.5ml
CXR Reference Dye	C541C	1 × 500μl	30μM	View Product
GoScript™ RT Mix for 1-Step RT-qPCR	M700C	1 × 500μl
Nuclease-Free Water	P119E	1 × 13ml		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What's in the box?

Item	Part #	Size	Concentration	Available Separately
MgCl₂	A351B	1 × 750μl	25mM	View Product
GoScript™ 5X Reaction Buffer	A500B	1 × 300μl		View Product
GoScript™ Reverse Transcriptase	A501B	1 × 50μl
GoTaq® Probe qPCR Master Mix, 2X	A610A	2 × 1ml
Oligo(dT)₁₅ Primer	C110B	1 × 50μg	500μg/ml	View Product
PCR Nucleotide Mix	C114G	1 × 200μl	10mM	View Product
Random Primers	C118B	1 × 50μg		View Product
CXR Reference Dye	C541A	1 × 100μl		View Product
Recombinant RNasin® Ribonuclease Inhibitor	N251A	1 × 2,500u	40u/μl	View Product
Nuclease-Free Water	P119A	3 × 1,250μl		View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

qPCR Probe Kits: GoTaq® Probe qPCR and RT-qPCR Systems

Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

Simple, Sensitive Probe-Based qPCR and RT-qPCR

Choose qPCR or RT-qPCR Master Mix Formats

Sensitivity and Specificity for qPCR

Reverse Transcription and qPCR in One Reaction

Two-Step Reverse Transcription and qPCR

Performance Tested in qPCR

Suitable for Different Organisms and Sample Types

Compatible with these Instruments

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Citations

Similar Products

GoTaq® qPCR and RT-qPCR Systems

Frequently Used With

M-MLV Reverse Transcriptase, RNase H Minus

PureYield™ Plasmid Maxiprep System

ReliaPrep™ RNA Miniprep Systems

FuGENE® SI Transfection Reagent

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