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E6110_ONE-Glo--Luciferase-Assay-System--10ml_3 E6120_ONE-Glo--Luciferase-Assay-System--100ml_3 E6130_ONE-Glo--Luciferase-Assay-System--1L_3

Robust Performance for High- and Ultrahigh-Throughput Reporter Assays

  • Less sensitive to mixing and dispensing conditions, enhancing reproducibility
  • Ideal for use in high-density (384- and 1536-well) microplates
  • Reduced odor and convenient storage options

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$ 177.00

Catalog Number: E6110

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$ 1,099.00

Catalog Number: E6120

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$ 8,414.00

Catalog Number: E6130

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Overview
Protocols
Specifications
Resources
Related Products
Citations

Not sure if this assay is right for you?
We have a product guide to help you choose the right Luciferase Reporter Assay.

View our Reporter Assay Comparison Guide

Bright, Stable Luminescence Signal for Batch and Continuous Processing Systems

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.

Assay Advantages

  • Room temperature or 4°C storage options. The extended stability of the ONE-Glo™ Reagent makes it more convenient for everyday use.
  • Improved Assay Precision. The ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, enhancing reproducibility. Ideal for use in high-density (384- and 1536-well) microplates.
  • Brighter, Longer-Lasting Signal. Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations.
  • Reduced Unwanted Effects from Sample Components. The ONE-Glo™ Assay is less sensitive to culture media, phenol red and luciferase inhibitors than other luciferase assays.
Extended bright light output allows high-sensitivity assays to be run in batch or continuous operations.
Extended bright light output allows high-sensitivity assays to be run in batch or continuous operations.

Extended Stability at 22°C

Reconstituted ONE-Glo Reagent can be used much longer than other bright-type reagents.
Reconstituted ONE-Glo™ Reagent can be used much longer than other bright-type reagents.

Phenol Red Stability

ONE-Glo Reagent is more tolerant of phenol red than other luciferin-based reagents.
ONE-Glo™ Reagent is more tolerant of phenol red than other luciferin-based reagents.

Specifications

Catalog Number:

What's in the box?

Item Part # Size

ONE-Glo™ Luciferase Assay Buffer

E605A 1 × 10ml

ONE-Glo™ Luciferase Assay Substrate

E606A 1 × 1 vial

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

What's in the box?

Item Part # Size

ONE-Glo™ Luciferase Assay Buffer

E605B 1 × 100ml

ONE-Glo™ Luciferase Assay Substrate

E606B 1 × 1 vial

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

What's in the box?

Item Part # Size

ONE-Glo™ Luciferase Assay Buffer

E6051 1 × 1L

ONE-Glo™ Luciferase Assay Substrate

E606C 1 × 1 vial

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Product Citations

Recent publications that mention the use of this product.

View PubMed Citations