Trypsin/Lys-C Mix, Mass Spec Grade

V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
Protein Cleavage with Trypsin-Lys-C
V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
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Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

$ 77.00
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This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

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This product is available under our Catalog (FT) program - Learn More
Trypsin/Lys-C Mix, Mass Spec Grade
20μg
$ 77.00
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Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Trypsin digest illustration.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protocols

Complete Protocol

Specifications

You are viewing: V5071 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 1 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5072 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507B 1 × 100μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5073 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 5 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Posters

Other Resources

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Frequently Used With

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Asp-N, Sequencing Grade

Cleavage site: N-terminal of Asp. Optimal pH: 4–9.

V1621

Chymotrypsin, Sequencing Grade

Cleavage sites: C-terminal of Tyr, Phe, Trp. Optimal pH: 7–9.

V1061, V1062

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