FastBreak™ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ Protein Purification System. This product is suitable for both manual and automated protocols.
Eliminate centrifugation and mechanical disruption with this easy-to-use, add and incubate reagent, compatible with manual and robotic platforms.