Viability PCR Reagent System
Improved Specific PCR Detection from Viable Cells and Intact Viruses
- Selectively amplify nucleic acid from viable cells, bacteria or viruses
- Faster and easier alternative to dyes or culture-based methods for viability
- Can be used ahead of dPCR or qPCR
- High-throughput compatible
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A Simple, Effective Method for Amplification-Based Live/Dead Discrimination
The Viability PCR Reagent System uses a proprietary molecule that irreversibly modifies nucleic acid so that it cannot be amplified. This molecule is cell- and capsid-impermeable, enabling selective detection of nucleic acid from only live cells or intact viruses. Treatment with the Viability PCR Reagent reduces false-positive amplification results caused by nucleic acid from compromised viruses or dead cells.
Amplification of DNA from heat-treated bacterial samples containing 103 CFU/ml treated with (+VR) or without (–VR) viability reagent.
Rapid Results of PCR with Ability to Discriminate Between Signal from Viable and Nonviable Cells and Viruses
The Viability PCR Reagent is added to sample and cannot enter intact cells or viral particles.
In contrast, the Viability PCR Reagent penetrates compromised cells (left) or viruses (right) and covalently modifies nucleic acid. As a result, PCR signal is only observed from nucleic acid from viable cells or viruses.
Live-Dead Discrimination with Bacteria
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Intact-Nonintact Discrimination with Viruses
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Suitable for a Variety of Viruses, Gram-Negative and Gram-Positive Bacteria in Diverse Matrices
Sample Types
- S. aureus
- P. aeruginosa
- E. coli
- Mycobacterium
- Listeria
- Legionella
- Probiotic cocktails
- Norovirus
- Hepatitis A
- AAV
DNA/RNA from organisms and sample types not listed also may be compatible.
Please enquire with Promega Technical Support for additional compatibility information.
Sample Matrices
- Cooling tower water
- Drinking water
- Biofilms
- Cocoa powder
- Pus
- Blood
- Cooling tower water
- Cocoa powder
Compatible with Most Nucleic Acid Purification Techniques and Amplification Protocols
Simply incubate sample with reagent, add neutralization buffer and proceed to nucleic acid extraction and amplification.
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Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Viability PCR Reagent |
AM230A | 1 × 250μl | 1mM |
Viability PCR Neutralization Buffer (10X) |
AM300A | 1 × 5ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Patent Pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Viability PCR Reagent |
AM220A | 1 × 50μl | 30mM |
Viability PCR Neutralization Buffer (10X) |
AM300A | 2 × 5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Patent Pending.
