Need a fit-for-purpose lyophilized PCR amplification assay? Learn more

M-MLV Reverse Transcriptase

M1701_M-MLV-Reverse-Transcriptase--10-000u_3
customize-this-small
View information on global supply logistics

RNA-Dependent DNA Polymerase for cDNA Synthesis

  • Enzyme supplied at a concentration of 200u/μl

Choose a product

Size

Catalog number selected: M1701

$ 87.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
M-MLV Reverse Transcriptase
Enzyme/10,000u
$ 87.00
Your price: Log in
Change Configuration

RNA-Dependent DNA Polymerase for cDNA Synthesis

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Advantages
  • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
  • May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
View M-MLV Citations

Protocols

Complete Protocol

Specifications

You are viewing: M1701 Change Configuration

What's in the box?

Item Part # Size

M-MLV Reverse Transcriptase

 M170A 1 × 10,000u

M-MLV RT 5X Buffer

 M531A 1 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: M1705 Change Configuration

What's in the box?

Item Part # Size

M-MLV Reverse Transcriptase

 M170B 1 × 50,000u

M-MLV RT 5X Buffer

 M531A 2 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: M5313 Change Configuration

What's in the box?

Item Part # Size

M-MLV RT 5X Buffer

 M531A 2 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Resources

Articles

Citations

Similar Products

Access RT-PCR System

System providing individual components for assembly of RT-PCR in a single tube.

A1260, A1250, A1280

Reverse Transcription System

Transcribes cDNA from RNA in as little as 15 minutes.

A3500

M-MLV Reverse Transcriptase, RNase H Minus

Helpful for preparing long cDNAs and libraries with a high percentage of full-length cDNA.

M5301

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Enzyme for cDNA synthesis from long (>5kb) RNA transcripts.

M3681, M3682, M3683

AMV Reverse Transcriptase

Reverse transcriptase with stability at higher reaction temperatures.

M5101, M5108, M9004

Frequently Used With

dNTP Mix

Premixed solution of dATP, dCTP, dGTP and dTTP in water.

U1511, U1515

Random Primers

Random hexamers for universal cDNA synthesis.

C1181

Oligo(dT)15 Primer

Primer for first-strand cDNA synthesis of mRNAs.

C1101

RNasin® Ribonuclease Inhibitor

The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases.

N2111, N2115, N2511, N2515, N2611, N2615

SV Total RNA Isolation System

Simple spin or vacuum-based method to extract total RNA from tissues, cultured cells and white blood cells.

Z3101, Z3100, Z3105

ReliaPrep™ FFPE Total RNA Miniprep System

Amplifiable total RNA from FFPE tissues without organic solvents or overnight digestion.

Z1001, Z1002

Maxwell® RSC simplyRNA Cells Kit

Purifies RNA from cells using a low elution volume and the Maxwell® RSC Instrument.

AS1390

Let's find the product that meets your needs.

Talk to a Scientist

Sabine

Sabine

Germany
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.