Taq DNA Polymerase Selection Guide
Guidelines to help you choose the right Taq formulation for your needs
Factors to consider when choosing a thermostable polymerase for your PCR include the intended application, enzyme characteristics, fidelity, reaction optimization needs and ease-of-use.
We offer a full range of high-quality Taq DNA polymerase formulations that include optimized enzymes and buffer systems for reliable, robust amplification. Taq DNA polymerase products include hot-start and standard PCR options, master mixes, and customizable buffer systems. We also offer Pfu DNA polymerase for applications requiring higher fidelity amplification.
Review the properties of the various Taq and Pfu formulations using the table below to choose the enzyme best suited to your research needs.
Compare thermostable DNA polymerase features
| Applications |
Standard PCRGoTaq® G2 DNA Polymerase |
Hot Start PCRGoTaq® G2 Hot Start DNA Polymerase |
Long Range PCRGoTaq® Long PCR Master Mix |
High Fidelity PCRPfu DNA Polymerase(not available in all countries) |
|---|---|---|---|---|
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High throughput |
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Long PCR |
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Cloning and subcloning |
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High-fidelity |
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Site-directed mutagenesis |
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Template generation for sequencing |
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Genotyping |
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Multiplex PCR |
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Colony PCR |
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Fast PCR |
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Routine PCR |
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| Characteristics |
Standard PCRGoTaq® G2 DNA Polymerase |
Hot Start PCRGoTaq® G2 Hot Start DNA Polymerase |
Long Range PCRGoTaq® Long PCR Master Mix |
High FidelityPfu DNA Polymerase(not available in all countries) |
|---|---|---|---|---|
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5´ - 3´ exonuclease activity |
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3´ - 5´ exonuclease activity |
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Amplicon Size |
<5kb |
<5kb |
<40kb |
<10kb |
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Enzyme type |
Recombinant |
Recombinant |
Recombinant |
Native |
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Product Overhang |
3´ A |
3´ A |
3´ A/ blunt |
blunt |
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Hot Start technology |
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| Formats |
Standard PCRGoTaq® G2 DNA Polymerase |
Hot Start PCRGoTaq® G2 Hot Start DNA Polymerase |
Long Range PCRGoTaq® Long PCR Master Mix |
High FidelityPfu DNA Polymerase(not available in all countries) |
|---|---|---|---|---|
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Enzyme with direct loading Buffer |
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Buffer with MgCl2 |
GoTaq® G2 Flexi DNA Polymerase |
GoTaq® G2 Hot Start DNA Polymerase |
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Contains MgSO4 |
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Master Mix with direct loading Buffer, dNTPs and enhancers (Green) |
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Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless) |
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Compare DNA polymerase features
GoTaq® G2 DNA Polymerase
Applications
Cloning and subcloning
High-fidelity
Site-directed mutagenesis
Template generation for sequencing
Genotyping
Multiplex PCR
Colony PCR
Fast PCR
Routine PCR
Characteristics
5´ - 3´ exonuclease activity
3´ - 5´ exonuclease activity
Amplicon Size
<5kb
Enzyme type
Recombinant
Product Overhang
3´ A
Hot Start technology
Formats
Enzyme with direct loading Buffer
Buffer with MgCl2
Buffer without MgCl2
GoTaq® G2 Flexi DNA Polymerase
Master Mix with direct loading Buffer, dNTPs and enhancers (Green)
GoTaq® G2 Green Master Mix
Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)
GoTaq® G2 Colorless Master Mix
GoTaq® G2 Hot Start DNA Polymerase
Applications
Cloning and subcloning
High-fidelity
Site-directed mutagenesis
Template generation for sequencing
Genotyping
Multiplex PCR
Colony PCR
Fast PCR
Routine PCR
Characteristics
5´ - 3´ exonuclease activity
3´ - 5´ exonuclease activity
Amplicon Size
<5kb
Enzyme type
Recombinant
Product Overhang
3´ A
Hot Start technology
Formats
Enzyme with direct loading Buffer
Buffer with MgCl2
Buffer without MgCl2
GoTaq® G2 Hot Start DNA Polymerase
Master Mix with direct loading Buffer, dNTPs and enhancers (Green)
GoTaq® G2 Hot Start Green Master Mix
Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)
GoTaq® G2 Hot Start Colorless Master Mix
GoTaq® Long PCR Master Mix
Applications
Cloning and subcloning
High-fidelity
Site-directed mutagenesis
Template generation for sequencing
Genotyping
Multiplex PCR
Colony PCR
Fast PCR
Routine PCR
Characteristics
5´ - 3´ exonuclease activity
3´ - 5´ exonuclease activity
Amplicon Size
<40kb
Enzyme type
Recombinant
Product Overhang
3´ A/ blunt
Hot Start technology
Formats
Enzyme with direct loading Buffer
Buffer with MgCl2
Buffer without MgCl2
Master Mix with direct loading Buffer, dNTPs and enhancers (Green)
Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)
Pfu DNA Polymerase
(not available in all countries)
Applications
Cloning and subcloning
High-fidelity
Site-directed mutagenesis
Template generation for sequencing
Genotyping
Multiplex PCR
Colony PCR
Fast PCR
Routine PCR
Characteristics
5´ - 3´ exonuclease activity
3´ - 5´ exonuclease activity
Amplicon Size
<10kb
Enzyme type
Native
Product Overhang
blunt
Hot Start technology
Formats
Enzyme with direct loading Buffer
Buffer with MgCl2
Buffer without MgCl2
Contains MgSO4
Master Mix with direct loading Buffer, dNTPs and enhancers (Green)
Master Mix without direct loading Buffer, dNTPs and enhancers (Colorless)
What are the Advantages of Hot-Start PCR?
- Hot start PCR reduces non-specific priming and primer dimer formation
- Antibody-mediated hot start technology allows room temperature reaction set-up
- Supports use in areas where room temperature may fluctuate
- Supports automated platforms setup
- Short 2-minute initial denature time at 94-95°C activates Taq DNA polymerase for amplification
- Antibodies have no effect on polymerase performance, so all enzyme features are intact as in the non-hot-start enzyme
- High specificity
