ECL Western Blotting Substrate

W1001_ECL-Western-Blotting-Substrate-250ml_3
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Chemiluminescent Substrate to Detect Horseradish Peroxidase (HRP) Conjugates on Immunoblots

  • Detect picogram levels of protein with minimal background
  • Non-radioactive
  • No optimization required; easily switch from other entry-level ECL substrates

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Catalog number selected: W1001

$ 205.00
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ECL Western Blotting Substrate
250ml
$ 205.00
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Highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.

No optimization required; easy to switch from other entry-level ECL substrates.

Applications
  • Western blotting
  • Dot blotting
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells. The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate, secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and with 1-minute exposure.

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: W1001 Change Configuration

What's in the box?

Item Part # Size

Peroxide Solution

 W100A 1 × 125ml

Luminol Enhancer Solution

 W101A 1 × 125ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Specifications

You are viewing: W1015 Change Configuration

What's in the box?

Item Part # Size

Peroxide Solution

 W100B 1 × 250ml

Luminol Enhancer Solution

 W101B 1 × 250ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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Secondary antibody that reacts with heavy and light chains of all subclasses of mouse IgG.

W4021

Anti-Human IgG (H+L), HRP Conjugate

Secondary antibody that reacts with heavy and light chains of all subclasses of human IgG.

W4031

Donkey Anti-Goat IgG, HRP

Secondary antibody that reacts with goat and sheep IgG.

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