Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.
The HaloTag® Succinimidyl Ester (O4) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (O4). The HaloTag® Succinimidyl Ester (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag® Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages (see figure). The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Amine (O4) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (O4). The HaloTag® Amine (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
The HaloTag® Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag® Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters (see figure), STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.
For more information, see the Protocols & Applications Guide.
References
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 139–40.
- Gretch, D.R., Suter, M. and Stinski, M.F. (1987) Anal. Biochem. 163, 270–77.
- Duthie, R.S. et al. (2002) Bioconjugate Chem. 13, 699–706.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 137–46.
- Gee, K.R. et al. (1999) Tetrahedron Letters 40, 1471–4.
- Adamczyk, M. et al. (1997) Bioconjugate Chem. 8, 253–5.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–8.
- Chen, J. and Selvin, P.R. (1999) Bioconjugate Chem. 10, 311–5.
- Ge, P. and Selvin, P.R. (2003) Bioconjugate Chem. 14, 870–76.
- Krokhin, O.V. et al. (2003) Biochemistry 42, 12950–59.
- Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–52.