pFC37K HiBiT CMV-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC37K HiBiT CMV-neo Flexi Vector sequence reference points: Base pairs 4654 CMV immediate-early enhancer/promoter 1-742 chimeric intron 857-989 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 SgfI Site 1056-1063 Barnase coding region 1087-1422 EcoICR I 1442-1447 HiBiT 1474-1506 Sv40 late polyadenylation signal 1643-1864 SV40 enhancer and early promoter 1963-2381 SV40 Enhancer 2036-2272 (Reverse) Sv40 Min Ori 2279-2344 EM7 bacterial promoter 2389-2455 Neo-Kan Resistance 2469-3263 Synthetic PolyAdenylation Signal Sequence 3327-3375 ColE1-derived plasmid replication origin 3611-3647 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCAAG 1451 GCAGTTCAGG TGGCTCGAGC GGTGTGAGCG GCTGGCGGCT GTTCAAGAAG 1501 ATTAGCTAAG GCCGCGACTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT 1551 CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC 1601 TGAGCAATAA CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG 1651 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 1701 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1751 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 1801 TTTCAGGTTC AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT 1851 CTACAAATGT GGTAAAATCG AATTTTAACA AAATATTAAC GCTTACAATT 1901 TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC 1951 ATACGCGGAT CTGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA 2001 ACTTGGTTAG GTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG 2051 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 2101 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 2151 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 2201 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 2251 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 2301 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 2351 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TAATTAACTG TTGACAATTA 2401 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT 2451 AAACCCAGGA GGCAGATCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 2501 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 2551 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 2601 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA 2651 GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 2701 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 2751 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 2801 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 2851 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 2901 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA 2951 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 3001 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 3051 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG 3101 GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 3151 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 3201 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA 3251 CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC 3301 GCCCAACCTG CCATCACGAT GGCCGCAATA AAATATCTTT ATTTTCATTA 3351 CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGCGATAA GGATCCTCTT 3401 TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC 3451 GGGGTCAGCA CCGTTTCTGC GGACTGGCTT TCTACCCGGT ATCAGCTCAC 3501 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 3551 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 3601 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 3651 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 3701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 3751 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 3801 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 3851 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 3901 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 3951 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 4001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 4051 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 4101 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 4151 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATTTC 4201 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 4251 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 4301 CTAGATCCTT TTATAGTCCG GAAATACAGG AACGCACGCT GGATGGCCCT 4351 TCGCTGGGAT GGTGAAACCA TGAAAAATGG CAGCTTCAGT GGATTAAGTG 4401 GGGGTAATGT GGCCTGTACC CTCTGGTTGC ATAGGTATTC ATACGGTTAA 4451 AATTTATCAG GCGCGATTGC GGCAGTTTTT CGGGTGGTTT GTTGCCATTT 4501 TTACCTGTCT GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG CGGTGCGTAC 4551 AATTAAGGGA TTATGGTAAA TCCACTTACT GTCTGCCCTC GTAGCCATCG 4601 AGATAAACCG CAGTACTCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG 4651 ATCT Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.