pBiT3.1-N [CMV/HiBiT/Blast] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pBiT3.1-N [CMV/HiBiT/Blast] Vector sequence reference points: Base pairs 4326 CMV promoter 276-866 chimeric intron 981-1113 T7 RNA Polymerase promoter (-17 to +3) 1157-1176 SgfI Site 1181-1188 HiBiT 1190-1225 EcoICR I 1228-1233 PmeI site 1316-1323 Sv40 late polyadenylation signal 1380-1601 EM7 bacterial promoter 1667-1733 Neo-Kan Resistance 1747-2541 ColE1-derived plasmid replication origin 2696-2732 Synthetic PolyAdenylation Signal Sequence 3413-3461 (Reverse) Blasticidin resistance (BlastR) coding region 3485-3883 (Reverse) Sv40 Min Ori 3945-4010 (Reverse) SV40 Enhancer 4017-4253 ---- 1 AAGACGCGTT AATTAAGACG TCGGCAGTGA AAAAAATGCT TTATTTGTGA 51 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 101 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 151 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 201 TGATTATGAT CCGCGGCCGC AATACGTCGA CGTTATCAGC TGACTTCGTA 251 CGAGAGCCTA GGATTATGGC GCGCCACTAG TTATTAATAG TAATCAATTA 301 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 351 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC 401 GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 451 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 501 CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA 551 ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA 601 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 651 TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 701 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA 751 ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA 801 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 851 AGTGAACCGT CAGATCACTA GAATCTTTAT TGCGGTAGTT TATCACAGTT 901 AAATTGCTAA CGCAGTCAGT GCTTCTGACA CAACAGTCTC GAACTTAAGC 951 TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AGGTTACAAG 1001 ACAGGTTTAA GGAGACCAAT AGAAACTGGG CTTGTCGAGA CAGAGAAGAC 1051 TCTTGCGTTT CTGATAGGCA CCTATTGGTC TTACTGACAT CCACTTTGCC 1101 TTTCTCTCCA CAGGTGTCCA CTCCCAGTTC AATTACAGCT CTTAAGGCTA 1151 GAGTATTAAT ACGACTCACT ATAGGGGTTA GCGATCGCCA TGGTGAGCGG 1201 CTGGCGGCTG TTCAAGAAGA TTAGCGGGAG CTCCGGTGGC TCGAGCGGTG 1251 GGAATTCTGG TGGAGGATCC GGTGGAGCTA GCGGAAGATC TGGTTCTAGA 1301 GGTCAAGCTT CGGGGGTTTA AACCTGCAGG CATGCAAGCT GATTTGGCTG 1351 CTGCCACCGC TGAGCAAGGC CGCTTCGAGC AGACATGATA AGATACATTG 1401 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT 1451 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA 1501 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG 1551 GGGAGATGTG GGAGGTTTTT TTAAGCAAGT AAAACCTCTA CAAATGTGGT 1601 AAAATCTTAA TTAAGACTTC TAATATAACT TCGTATAGCA TACATTATAC 1651 GAAGTTATGG TTCCACTGTT GACAATTAAT CATCGGCATA GTATATCGGC 1701 ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCCAGGAGG CAGATCATGA 1751 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 1801 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC 1851 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 1901 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 1951 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 2001 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 2051 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 2101 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA 2151 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 2201 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 2251 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 2301 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 2351 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 2401 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA 2451 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 2501 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG ATTCGAAATG 2551 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA 2601 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 2651 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 2701 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 2751 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 2801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 2851 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 2901 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 2951 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 3001 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 3051 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 3101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 3151 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 3201 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 3251 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA 3301 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 3351 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA 3401 GTCCGGAATC GTCACACAAA AAACCAACAC ACAGATGTAA TGAAAATAAA 3451 GATATTTTAT TGCGGCCATC GTGATGGCTA GAGTTTAGCC CTCCCACACA 3501 TAACCAGAGG GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC 3551 ACTGTCCTTC ACTATGGCTT TGATCCCAGG ATGCAGATCG AGAAGCACCT 3601 GTCGGCACCG TCCGCAGGGG CTCAAGATGC CCCTGTTCTC ATTTCCGATC 3651 GCGACGATAC AAGTCAGGTT GCCAGCTGCC GCAGCAGCAG CAGTGCCCAG 3701 CACCACGAGT TCTGCACAAG GTCCCCCAGT AAAATGATAT ACATTGACAC 3751 CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC GACGCTGTAG 3801 TCTTCAGAGA TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG 3851 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGTGGCG CTAGTGTCAG 3901 AAGAATCGAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC 3951 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 4001 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG 4051 TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA 4101 TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 4151 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 4201 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC 4251 AGCTGGTTCT TTCCGCCTCA GAAGGTACCT AACCAAGTTC CTCTTTCAGA 4301 GGTTATTTCA GGCCATGGTG CTGCGC Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.