pBiT3.1-C [CMV/HiBiT/Blast] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pBiT3.1-C [CMV/HiBiT/Blast] Vector sequence reference points: Base pairs 4323 CMV promoter 276-866 chimeric intron 981-1113 T7 RNA Polymerase promoter (-17 to +3) 1157-1176 SgfI Site 1177-1184 EcoICR I 1252-1257 HiBiT 1262-1294 PmeI site 1298-1305 Sv40 late polyadenylation signal 1377-1598 EM7 bacterial promoter 1664-1730 Neo-Kan Resistance 1744-2538 ColE1-derived plasmid replication origin 2693-2729 Synthetic PolyAdenylation Signal Sequence 3410-3458 (Reverse) Blasticidin resistance (BlastR) coding region 3482-3880 (Reverse) Sv40 Min Ori 3942-4007 (Reverse) SV40 Enhancer 4014-4250 ---- 1 AAGACGCGTT AATTAAGACG TCGGCAGTGA AAAAAATGCT TTATTTGTGA 51 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 101 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 151 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 201 TGATTATGAT CCGCGGCCGC AATACGTCGA CGTTATCAGC TGACTTCGTA 251 CGAGAGCCTA GGATTATGGC GCGCCACTAG TTATTAATAG TAATCAATTA 301 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 351 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC 401 GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 451 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 501 CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA 551 ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA 601 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 651 TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 701 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA 751 ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA 801 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 851 AGTGAACCGT CAGATCACTA GAATCTTTAT TGCGGTAGTT TATCACAGTT 901 AAATTGCTAA CGCAGTCAGT GCTTCTGACA CAACAGTCTC GAACTTAAGC 951 TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AGGTTACAAG 1001 ACAGGTTTAA GGAGACCAAT AGAAACTGGG CTTGTCGAGA CAGAGAAGAC 1051 TCTTGCGTTT CTGATAGGCA CCTATTGGTC TTACTGACAT CCACTTTGCC 1101 TTTCTCTCCA CAGGTGTCCA CTCCCAGTTC AATTACAGCT CTTAAGGCTA 1151 GAGTATTAAT ACGACTCACT ATAGGGGCGA TCGCAACAAG CTTCGGGAAG 1201 ATCTGGAGCT AGCGGCGGGG GATCCGGTGG GAATTCTGGC TCGAGCGGTG 1251 GGAGCTCCGG TGTGAGCGGC TGGCGGCTGT TCAAGAAGAT TAGCTAAGTT 1301 TAAACGGCCG CGACTCTAGA CTGCAGGCAT GCAAGCTGAT TTGGCTGCTG 1351 CCACCGCTGA GCAAGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG 1401 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT 1451 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA 1501 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG 1551 AGATGTGGGA GGTTTTTTTA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA 1601 ATCTTAATTA AGACTTCTAA TATAACTTCG TATAGCATAC ATTATACGAA 1651 GTTATGGTTC CACTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA 1701 GTATAATACG ACAAGGTGAG GAACTAAACC CAGGAGGCAG ATCATGATTG 1751 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 1801 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 1851 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 1901 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 1951 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 2001 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 2051 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 2101 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 2151 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 2201 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 2251 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 2301 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 2351 TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 2401 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 2451 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 2501 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGATT CGAAATGACC 2551 GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 2601 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 2651 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 2701 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 2751 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 2801 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 2851 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 2901 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 2951 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 3001 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 3051 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 3101 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 3151 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 3201 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 3251 GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT CCTTTGATCT 3301 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 3351 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTATAGTC 3401 CGGAATCGTC ACACAAAAAA CCAACACACA GATGTAATGA AAATAAAGAT 3451 ATTTTATTGC GGCCATCGTG ATGGCTAGAG TTTAGCCCTC CCACACATAA 3501 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT 3551 GTCCTTCACT ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC 3601 GGCACCGTCC GCAGGGGCTC AAGATGCCCC TGTTCTCATT TCCGATCGCG 3651 ACGATACAAG TCAGGTTGCC AGCTGCCGCA GCAGCAGCAG TGCCCAGCAC 3701 CACGAGTTCT GCACAAGGTC CCCCAGTAAA ATGATATACA TTGACACCAG 3751 TGAAGATGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT 3801 TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT CAATGAGGGT 3851 GGATTCTTCT TGAGACAAAG GCTTGGCCAT GGTGGCGCTA GTGTCAGAAG 3901 AATCGAGCTT TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC 3951 TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 4001 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA 4051 GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG 4101 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 4151 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG 4201 CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC 4251 TGGTTCTTTC CGCCTCAGAA GGTACCTAAC CAAGTTCCTC TTTCAGAGGT 4301 TATTTCAGGC CATGGTGCTG CGC Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.