pFN39K secHiBiT CMV-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN39K secHiBiT CMV-neo Flexi Vector sequence reference points: Base pairs 4747 CMV immediate-early enhancer/promoter 1-742 chimeric intron 857-989 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 IL6 Signal Sequence 1065-1151 HiBiT 1152-1184 EcoICR I 1187-1192 SgfI Site 1209-1216 Barnase coding region 1240-1575 PmeI site 1577-1584 Sv40 late polyadenylation signal 1736-1957 SV40 enhancer and early promoter 2056-2474 SV40 Enhancer 2129-2365 (Reverse) Sv40 Min Ori 2372-2437 EM7 bacterial promoter 2482-2548 Neo-Kan Resistance 2562-3356 Synthetic PolyAdenylation Signal Sequence 3420-3468 ColE1-derived plasmid replication origin 3704-3740 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGAAC TCCTTCTCCA CAAGCGCCTT CGGTCCAGTT 1101 GCCTTCTCCC TGGGCCTGCT CCTGGTGTTG CCTGCTGCCT TCCCTGCCCC 1151 AGTGAGCGGC TGGCGGCTGT TCAAGAAGAT TAGCGGGAGC TCCGGTGGCT 1201 CGAGCGGTGC GATCGCCATG GAATAAGTAA GGAATCCACA TGGCACAGGT 1251 TATCAACACG TTTGACGGGG TTGCGGATTA TCTTCAGACA TATCATAAGC 1301 TACCTGATAA TTACATTACA AAATCAGAAG CACAAGCCCT CGGCTGGGTG 1351 GCATCAAAAG GGAACCTTGC AGACGTCGCT CCGGGGAAAA GCATCGGCGG 1401 AGACATCTTC TCAAACAGGG AAGGCAAACT CCCGGGCAAA AGCGGACGAA 1451 CATGGCGTGA AGCGGATATT AACTATACAT CAGGCTTCAG AAATTCAGAC 1501 CGGATTCTTT ACTCAAGCGA CTGGCTGATT TACAAAACAA CGGACCATTA 1551 TCAGACCTTT ACAAAAATCA GATAATGTTT AAACGAATTC GGGCTCGGTA 1601 CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT GATCCGGCTG 1651 CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA 1701 TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 1751 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC 1801 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG 1851 CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 1901 TTCAGGGGGA GATGTGGGAG GTTTTTTTAA GCAAGTAAAA CCTCTACAAA 1951 TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA ATTTCCTGAT 2001 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 2051 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT 2101 TAGGTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA 2151 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 2201 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG 2251 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 2301 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 2351 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 2401 CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 2451 GGCCTAGGCT TTTGCAAAAA GCTTAATTAA CTGTTGACAA TTAATCATCG 2501 GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCCA 2551 GGAGGCAGAT CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 2601 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 2651 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 2701 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 2751 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 2801 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG 2851 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 2901 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 2951 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 3001 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 3051 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA 3101 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA 3151 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 3201 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 3251 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 3301 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 3351 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC 3401 CTGCCATCAC GATGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT 3451 GTGTTGGTTT TTTGTGTGAA TCGATAGCGA TAAGGATCCT CTTTGCGCTT 3501 GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTCA 3551 GCACCGTTTC TGCGGACTGG CTTTCTACCC GGTATCAGCT CACTCAAAGG 3601 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 3651 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 3701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 3751 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 3801 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 3851 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 3901 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 3951 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 4001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 4051 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 4101 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 4151 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 4201 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 4251 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT TTCAAGAAGA 4301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 4351 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 4401 CTTTTATAGT CCGGAAATAC AGGAACGCAC GCTGGATGGC CCTTCGCTGG 4451 GATGGTGAAA CCATGAAAAA TGGCAGCTTC AGTGGATTAA GTGGGGGTAA 4501 TGTGGCCTGT ACCCTCTGGT TGCATAGGTA TTCATACGGT TAAAATTTAT 4551 CAGGCGCGAT TGCGGCAGTT TTTCGGGTGG TTTGTTGCCA TTTTTACCTG 4601 TCTGCTGCCG TGATCGCGCT GAACGCGTTT TAGCGGTGCG TACAATTAAG 4651 GGATTATGGT AAATCCACTT ACTGTCTGCC CTCGTAGCCA TCGAGATAAA 4701 CCGCAGTACT CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCT Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.