pBiT3.1-secN [CMV/HiBiT/Blast] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pBiT3.1-secN [CMV/HiBiT/Blast] Vector sequence reference points: Base pairs 4410 CMV promoter 276-866 chimeric intron 981-1113 T7 RNA Polymerase promoter (-17 to +3) 1157-1176 SgfI Site 1181-1188 IL6 Signal Sequence 1190-1276 HiBiT 1277-1309 EcoICR I 1312-1317 PmeI site 1400-1407 Sv40 late polyadenylation signal 1464-1685 EM7 bacterial promoter 1751-1817 Neo-Kan Resistance 1831-2625 ColE1-derived plasmid replication origin 2780-2816 Synthetic PolyAdenylation Signal Sequence 3497-3545 (Reverse) Blasticidin resistance (BlastR) coding region 3569-3967 (Reverse) Sv40 Min Ori 4029-4094 (Reverse) SV40 Enhancer 4101-4337 ---- 1 AAGACGCGTT AATTAAGACG TCGGCAGTGA AAAAAATGCT TTATTTGTGA 51 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 101 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 151 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 201 TGATTATGAT CCGCGGCCGC AATACGTCGA CGTTATCAGC TGACTTCGTA 251 CGAGAGCCTA GGATTATGGC GCGCCACTAG TTATTAATAG TAATCAATTA 301 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 351 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC 401 GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 451 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 501 CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA 551 ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA 601 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 651 TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 701 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA 751 ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA 801 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 851 AGTGAACCGT CAGATCACTA GAATCTTTAT TGCGGTAGTT TATCACAGTT 901 AAATTGCTAA CGCAGTCAGT GCTTCTGACA CAACAGTCTC GAACTTAAGC 951 TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AGGTTACAAG 1001 ACAGGTTTAA GGAGACCAAT AGAAACTGGG CTTGTCGAGA CAGAGAAGAC 1051 TCTTGCGTTT CTGATAGGCA CCTATTGGTC TTACTGACAT CCACTTTGCC 1101 TTTCTCTCCA CAGGTGTCCA CTCCCAGTTC AATTACAGCT CTTAAGGCTA 1151 GAGTATTAAT ACGACTCACT ATAGGGGTTA GCGATCGCCA TGAACTCCTT 1201 CTCCACAAGC GCCTTCGGTC CAGTTGCCTT CTCCCTGGGC CTGCTCCTGG 1251 TGTTGCCTGC TGCCTTCCCT GCCCCAGTGA GCGGCTGGCG GCTGTTCAAG 1301 AAGATTAGCG GGAGCTCCGG TGGCTCGAGC GGTGGGAATT CTGGTGGAGG 1351 ATCCGGTGGA GCTAGCGGAA GATCTGGTTC TAGAGGTCAA GCTTCGGGGG 1401 TTTAAACCTG CAGGCATGCA AGCTGATTTG GCTGCTGCCA CCGCTGAGCA 1451 AGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC 1501 CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG 1551 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC 1601 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT 1651 TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC TTAATTAAGA 1701 CTTCTAATAT AACTTCGTAT AGCATACATT ATACGAAGTT ATGGTTCCAC 1751 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 1801 AGGTGAGGAA CTAAACCCAG GAGGCAGATC ATGATTGAAC AAGATGGATT 1851 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 1901 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 1951 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 2001 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG 2051 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 2101 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT 2151 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 2201 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 2251 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 2301 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC 2351 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 2401 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT 2451 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 2501 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 2551 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 2601 TCGCCTTCTT GACGAGTTCT TCTGATTCGA AATGACCGAC CAAGCGACGC 2651 CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 2701 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 2751 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 2801 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 2851 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 2901 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 2951 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 3001 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 3051 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 3101 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 3151 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 3201 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 3251 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 3301 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 3351 CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 3401 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 3451 TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG AATCGTCACA 3501 CAAAAAACCA ACACACAGAT GTAATGAAAA TAAAGATATT TTATTGCGGC 3551 CATCGTGATG GCTAGAGTTT AGCCCTCCCA CACATAACCA GAGGGCAGCA 3601 ATTCACGAAT CCCAACTGCC GTCGGCTGTC CATCACTGTC CTTCACTATG 3651 GCTTTGATCC CAGGATGCAG ATCGAGAAGC ACCTGTCGGC ACCGTCCGCA 3701 GGGGCTCAAG ATGCCCCTGT TCTCATTTCC GATCGCGACG ATACAAGTCA 3751 GGTTGCCAGC TGCCGCAGCA GCAGCAGTGC CCAGCACCAC GAGTTCTGCA 3801 CAAGGTCCCC CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC 3851 GTCGCTAGAG AGAGCTGCGC TGGCGACGCT GTAGTCTTCA GAGATGGGGA 3901 TGCTGTTGAT TGTAGCCGTT GCTCTTTCAA TGAGGGTGGA TTCTTCTTGA 3951 GACAAAGGCT TGGCCATGGT GGCGCTAGTG TCAGAAGAAT CGAGCTTTTT 4001 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT 4051 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG 4101 CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 4151 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT 4201 GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT 4251 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 4301 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC 4351 CTCAGAAGGT ACCTAACCAA GTTCCTCTTT CAGAGGTTAT TTCAGGCCAT 4401 GGTGCTGCGC Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.