Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates
Fluorescent Antibodies for Direct Detection of HiBiT-Tagged Proteins
- Bright signal from fluorescent dyes with the same structure as Alexa Fluor® dyes
- Streamline flow cytometry workflows
- Preserve samples with fewer wash steps
Catalog Number:
Choose a Wavelength
Size
Catalog Number: CS3278A02
Catalog Number: CS3278A06
Simplify Flow Cytometry for HiBiT-Tagged Proteins
Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates (same structure as Alexa Fluor® dyes), make flow cytometry analysis of HiBiT-tagged proteins more efficient. They eliminate the need for secondary antibodies, reducing long incubation times and wash steps that cause sample loss. Available in two different colors to enable multiplexing in both live-cell and fixed flow cytometry applications.
The 11aa HiBiT epitope tag provides researchers the flexibility to study protein dynamics in live cells, fixed cells and lysates with both sensitive bioluminescence detection and a high-affinity antibody. Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates, further enhance the versatile HiBiT protein tagging system.
Detect HiBiT CRISPR Knock-In Cells via Flow Cytometry
HiBiT knock-in (orange) and parental control cells (red) were stained with 1µg/ml of FarRed647 (top row) or Green488 (bottom row) Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates. Unstained HiBiT knock-in cells were included for comparison (blue). Samples were analyzed on a BD Accuri C6 Plus Flow Cytometer using the APC or FITC filters, respectively.
Alexa Fluor is a registered trademark of Thermo Fisher Scientific.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Anti-HiBiT Monoclonal Antibody—Green488 |
CS3278A01 | 1 × 100μl |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to
- (1) use in further material manufacture; and
- (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Anti-HiBiT Monoclonal Antibody—FarRed647 |
CS3278A05 | 1 × 100μl |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to
- (1) use in further material manufacture; and
- (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
Related Products
Similar Products
Anti-HiBiT Monoclonal Antibody
Specific and highly sensitive monoclonal antibody enabling Ab-based methods for HiBiT tag detection.
N7200, N7210
Anti-HiBiT Magnetic Beads and Elution Peptide
Fast and convenient HiBiT-tagged protein enrichment.
CS3278A08, CS3278A10, CS3002A02
Nano-Glo® HiBiT Blotting System
Luminescent method for rapid detection of HiBiT-tagged proteins on blots—no antibodies needed.
N2410
Frequently Used With
Nano-Glo® HiBiT Extracellular Detection System
A luminescent live-cell method to detect HiBiT-tagged proteins on the cell surface or secreted into the medium.
N2420, N2421, N2422
Nano-Glo® HiBiT Lytic Detection System
Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell.
N3030, N3040, N3050
Nano-Glo® HiBiT Dual-Luciferase® Reporter System
Sequential measurement of firefly luciferase and HiBiT-tagged proteins from the same sample in an “add-read-add-read” assay format.
CS1956A08, CS1956A09, CS1956B08, CS1956B09, CS1956B10, CS1956B11, CS1956B12, CS1956B13
Nano-Glo® Live Cell Assay
Measures NanoBiT® or NanoLuc® luminescence in living cells for up to 2 hours.
N2011, N2012, N2013
