ViaScript™ LgBiT mRNA Delivery System
- Improved live-cell, kinetic analysis of HiBiT-tagged proteins
- Efficient and uniform expression across cell population
- Faster expression than DNA plasmids to get results sooner
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Quantify Endogenous Protein Expression in Real Time
HiBiT, an 11-amino-acid epitope tag, spontaneously interacts with LgBiT to generate the bright NanoBiT® luciferase. Introduction of HiBiT into target gene loci with CRISPR/Cas9 gene editing allows the study of protein under endogenous expression. Kinetically quantify HiBiT-tagged proteins in live cells from a single well using ViaScript™ LgBiT mRNA Delivery System and Nano-Glo® Extended Live Cell Substrates.
Benefits of Kinetic HiBiT Detection
- Observe effects missed by a single time point (e.g., hook effect).
- Accurately determine key compound characteristics, such as protein degradation/recovery rates, allowing therapeutic discovery, triage and optimization.
- Achieve more reproducible results by sampling the same well over time.
- Save time and reagents by not needing separate wells for each time point.
ViaScript™ LgBiT mRNA Delivery System offers faster and more uniform LgBiT expression to get more representative and precise live-cell kinetic HiBiT assay results.
Achieve Uniform Expression for Consistent and Reliable Results
ViaScript™ LgBiT mRNA Delivery System provides more uniform and efficient delivery of LgBiT, allowing you to study your HiBiT-tagged protein of interest at endogenous levels in more biologically relevant cell lines.
A 22Rv1 AR-HiBiT clonal cell line was transfected with ViaScript™ LgBiT mRNA Delivery System (left) or LgBiT Expression Vector (right) overnight. Cells were treated for 6 hours with either a vehicle control (top) or 1µM ARV-110 PROTAC (bottom) to induce degradation of AR. Images were collected after addition of Nano-Glo® Live Cell Substrate on the GloMax® Galaxy.
Observe Effects Missed by Endpoint Assays
ViaScript™ LgBiT mRNA Delivery System enhances live-cell quantitation of protein levels in HiBiT CRISPR knock-in cell lines, offering deeper insights into models and compounds beyond endpoint assays. As shown above, kinetic HiBiT targeted protein degradation assays allow precise measurement of protein degradation rates, identification of hook effects (middle panel arrow) and characterization of protein recovery.
Get Results Faster
HiBiT signal peaks earlier after transfection with ViaScript™ LgBiT mRNA Delivery System than with DNA plasmid transfection, allowing you to start your experiments sooner and get data faster.
Perform Assays in More Biologically Relevant Cell Lines
The ViaScript™ LgBiT mRNA Delivery System delivers high transfection efficiency with minimal cell toxicity, outperforming DNA transfection across various cell lines. By pairing mRNA transfection with HiBiT CRISPR knock-in cell lines, you can work in cellular contexts that more accurately model patient biology, yielding more representative results and advancing research success.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
eGFP mRNA |
EV495A | 1 × 100μl | |
ViaScript™ Buffer |
EV100A | 1 × 11ml | |
ViaScript™ Reagent |
EV496A | 1 × 0.1ml | 1mg/ml |
LgBiT mRNA |
N421A | 1 × 100μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoLuc® and NanoBiT® Reporter mRNAs Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. Notwithstanding the foregoing, researcher may use this product in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the product. Researcher has no right to reverse engineer this product.
In addition, researcher must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 9,797,889 and other patents pending.
Patent Pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
ViaScript™ Buffer |
EV100A | 1 × 11ml | |
ViaScript™ Reagent |
EV496A | 1 × 0.1ml | 1mg/ml |
LgBiT mRNA |
N421A | 1 × 100μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoLuc® and NanoBiT® Reporter mRNAs Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. Notwithstanding the foregoing, researcher may use this product in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the product. Researcher has no right to reverse engineer this product.
In addition, researcher must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 9,797,889 and other patents pending.
Patent Pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
ViaScript™ Buffer |
EV100A | 5 × 11ml | |
ViaScript™ Reagent |
EV496A | 5 × 0.1ml | 1mg/ml |
LgBiT mRNA |
N421A | 5 × 100μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoLuc® and NanoBiT® Reporter mRNAs Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. Notwithstanding the foregoing, researcher may use this product in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the product. Researcher has no right to reverse engineer this product.
In addition, researcher must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 9,797,889 and other patents pending.
Patent Pending.
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