Rabbit Reticulocyte Lysate, Nuclease-Treated

L4960_Rabbit Reticulocyte Lysate- Nuclease-Treated_3
customize-this-small
View information on global supply logistics

In Vitro Translation of mRNA and Characterization of mRNA Products

  • Optimized for translation and ready to use
  • Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate

Size

Catalog number selected: L4960

$ 256.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Rabbit Reticulocyte Lysate, Nuclease-Treated
30 reactions
$ 256.00
Your price: Log in

Identify mRNAs, Characterize Protein Products 

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.

Choose Your Configuration: Learn more about custom options for this product at: www.promega.com/custom/

Applications
  • In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
  • Characterization of mRNA products
  • Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
References
  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
  2. Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Reticulocyte Lysate, Nuclease Treated

 L416A 5 × 200μl

Amino Acid Mixture Minus Cysteine

 L447C 1 × 50μl 1mM

Luciferase Control RNA

 L456A 1 × 10μl View Product

Amino Acid Mixture Minus Leucine

 L995A 1 × 50μl 1mM

Amino Acid Mixture Minus Methionine

 L996A 1 × 50μl 1mM View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Resources

Articles

Citations

Similar Products

Flexi® Rabbit Reticulocyte Lysate System

Translation reaction optimization for Mg2+ and K+ concentrations and option to add DTT. Endogenous Mg2+ concentration of each lysate batch is provided to aid in optimizing reactions.

L4540

TnT® Starter Bundle

Use for in vitro analysis of protein:protein or protein:nucleic acid interactions or to verify the ability of your clone to express protein.

L1215

Luciferase Control RNA

Unique functional control for in vitro translation reactions. Uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail producing functional luciferase when translated. Control reactions monitored by a luciferase assay.

L4561

Frequently Used With

Luciferase Control RNA

Unique functional control for in vitro translation reactions. Uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail producing functional luciferase when translated. Control reactions monitored by a luciferase assay.

L4561

Transcend™ Non-Radioactive Translation Detection System

Non-radioactive detection of proteins synthesized in vitro.

L5070

FluoroTect™ GreenLys in vitro Translation Labeling System

Fluorescent labeling and detection of in vitro synthesized proteins.

L5001

Magnesium Acetate

25mM magnesium acetate.

L4581

Potassium Chloride

2.5M solution to supplement TnT® reactions.

L4591

Let's find the product that meets your needs.

Talk to a Scientist

Alessandro

Alessandro

Italy
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.