Rabbit Reticulocyte Lysate, Nuclease-Treated

L4960_Rabbit Reticulocyte Lysate- Nuclease-Treated_3
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In Vitro Translation of mRNA and Characterization of mRNA Products

  • Optimized for translation and ready to use
  • Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate

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Catalog number selected: L4960

$ 256.00
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Rabbit Reticulocyte Lysate, Nuclease-Treated
30 reactions
$ 256.00
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Identify mRNAs, Characterize Protein Products 

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.

Choose Your Configuration: Learn more about custom options for this product at: www.promega.com/custom/

Applications
  • In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
  • Characterization of mRNA products
  • Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
References
  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
  2. Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Reticulocyte Lysate, Nuclease Treated

 L416A 5 × 200μl

Amino Acid Mixture Minus Cysteine

 L447C 1 × 50μl 1mM

Luciferase Control RNA

 L456A 1 × 10μl View Product

Amino Acid Mixture Minus Leucine

 L995A 1 × 50μl 1mM

Amino Acid Mixture Minus Methionine

 L996A 1 × 50μl 1mM View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Resources

Articles

Citations

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Unique functional control for in vitro translation reactions. Uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail producing functional luciferase when translated. Control reactions monitored by a luciferase assay.

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Frequently Used With

Luciferase Control RNA

Unique functional control for in vitro translation reactions. Uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail producing functional luciferase when translated. Control reactions monitored by a luciferase assay.

L4561

Transcend™ Non-Radioactive Translation Detection System

Non-radioactive detection of proteins synthesized in vitro.

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FluoroTect™ GreenLys in vitro Translation Labeling System

Fluorescent labeling and detection of in vitro synthesized proteins.

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Magnesium Acetate

25mM magnesium acetate.

L4581

Potassium Chloride

2.5M solution to supplement TnT® reactions.

L4591

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