Rabbit Reticulocyte Lysate, Untreated

L4151_Rabbit-Reticulocyte-Lysate--Untreated_3
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Characterize Protein Products, Study Transcriptional and Translational Control

  • Contains tRNA, ribosomes, amino acids, initiation, elongation and termination factors needed for protein synthesis
  • No micrococcal nuclease treatment
  • Consistent reticulocyte production from every lot

Size

Catalog number selected: L4151

$ 183.00
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Rabbit Reticulocyte Lysate, Untreated
1ml
$ 183.00
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Rabbit Reticulocyte Lysate, Untreated

Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates with the exception that it is not treated with micrococcal nuclease.

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Applications
  • Isolation of the cellular components necessary for protein synthesis.
  • Translation of exogenous mRNA, after further optimization, including the addition of energy-regenerating systems, hemin, endogenous tRNA and micrococcal nuclease to remove endogenous mRNA.
Reference
  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.

Protocols

Complete Protocol

Specifications

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Item Part # Size

Rabbit Reticulocyte Lysate, Untreated

 L415A 1 × 1,000μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Resources

Citations

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