NanoBRET® Protein Interaction Assays

We have developed NanoBRET® Protein:Protein Interaction (PPI) Assays to measure the interaction of specific protein pairs for a variety of biological targets. Assays are available by direct order from our catalog or by custom order via quote. Click on the catalog number to visit the product catalog page or check the box next to assays of interest to submit an inquiry for more information.

• RAS Signaling Collection

  Name
  BRAF/BRAF Dimer
  CRAF/CRAF Dimer
  HRasWT/CRAF FL or CRAF RBD
  KRasG12C/BRAF FL or BRAF RBD
  KRasG12D/ARAF FL or ARAF RBD
  KRasG12D/CRAF FL, CRAF RBD or CRAF RBD-CRD
  KRasG12V/BRAF FL or BRAF RBD
  KRasWT/ARAF FL or ARAF RBD
  KRasWT/CRAF FL, CRAF RBD or CRAF RBD-CRD
  NRasQ61K/CRAF FL or CRAF RBD
  NRasQ61R/CRAF FL or CRAF RBD
  NRasWT/CRAF FL or CRAF RBD

  Name
  BRAF/CRAF
  HRasWT/BRAF FL or BRAF RBD
  KRasG12C/ARAF FL or ARAF RBD
  KRasG12C/CRAF FL, CRAF RBD or CRAF RBD-CRD
  KRasG12D/BRAF or BRAF RBD
  KRasG12V/ARAF FL or ARAF RBD
  KRasG12V/CRAF FL, CRAF RBD or CRAF RBD-CRD
  KRasWT/BRAF FL or BRAF RBD
  NRasQ61K/BRAF FL or BRAF RBD
  NRasQ61R/BRAF FL or BRAF RBD
  NRasWT/BRAF FL or BRAF RBD

• Signaling Proteins and Kinase Assays

  Name
  β-catenin/BCL9L
  β-catenin/TCF4
  ERK/ELK
  GADD45B/MKK7
  MEK/ERK
  MycN/AURKA
  SHN3/ERK2
  SKP2/CKS1B

  Name
  β-catenin/TCF3
  cMyc/AURKA
  FKBP/FRB
  MAVS/IFIH1
  MID1/Alpha4
  SHN3/ERK1
  SHN3/SHN3 Dimer

• Other Epigenetic Assays

  Name
  CBX2/H3.1
  CBX7/Histone H3.2
  EZH2/SUZ12
  HDAC1/HDAC2
  MLL1/Menin
  PRMT5/RIOK1
  TUDOR/Histone H3.3

  Name
  CBX7/Histone H3.1
  CBX7/Histone H3.3
  FANCD2/Ubiquitin
  KDM4B/Histone H3.3
  MLL1/WDR5
  PRMT5/WDR77(MEP50)

• Transcriptional Protein Assays

  Name	Catalog #
  NanoBRET® cMyc/MAX Interaction Assay	N1870
  NanoBRET® PPI Control Pair (p53/MDM2)	N1641

  
  
  

  Name
  cMYc/WRD5
  FOXP3/FOXP3
  GR/GR (Dimerization)
  SP140/Histone H3.3
  TEAD1/YAP1
  TEAD2/YAP1
  TEAD3/YAP1
  TEAD4/YAP1

  Name
  ER/ER(Dimerization)
  FOXP3/NFATC2
  IRF5/IRF5 Dimer
  TEAD1/TAZ
  TEAD2/TAZ
  TEAD3/TAZ
  TEAD4/TAZ

• Membrane Protein Assays

  Name
  DAP12/Syk
  GRB2/EGFR
  IL17RA/ACT1
  MERTK dimer

  Name
  DAP12/TREM2
  HCN1/TRIP8b
  MET/Grb2
  STIM1/Orai

• Cellular Regulation

  Name
  ABHD5/PLIN5
  MCL-1/BIML

  Name
  CALR/MPL
  PNPLA3/ABHD5

• Protein Degradation

  Name
  BRD4/CRBN

  Name
  E6/E6AP

• Bromodomain Assays

  Name	Catalog #
  NanoBRET® BRD 4/Histone H3.3 Interaction Assay	N1830

  
  
  

  Name
  ATAD2/Histone H3.3
  BRD2/Histone H3.3
  BRD3/Histone H3.3
  BRD4 BD2/Histone H3.3
  BRD7 BD/Histone H3.3
  CBP/Histone H4
  SMARCA2/Histone H3.3

  Name
  ATAD2/Histone H4
  BRD2/Histone H4
  BRD3/Histone H4
  BRD4 BD2/Histone H4
  CBP/H3.3
  EP300 BD/Histone H4.4