Anti-HiBiT Magnetic Beads and Elution Peptide
For Convenient Affinity Purification of HiBiT-Tagged Proteins
- For immunoaffinity-based enrichment of HiBiT-tagged proteins
- High-affinity antibodies ensure tag-specific binding with minimal non-specific background
- Enables study of protein:protein interactions by Western blot or mass spectrometry
- DrkBiT peptide enables gentle elution of HiBiT-tagged proteins
Catalog Number:
Choose Magne® Beads or Elution Peptide
Size
Catalog Number: CS3278A08
Catalog Number: CS3278A10
Catalog Number: CS3002A02
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Study Protein:Protein Interactions Using HiBiT Tag
The HiBiT epitope tag provides a simple method for protein enrichment. The tag's compatibility with a sensitive and specific anti-HiBiT monoclonal antibody assists in effective immunocapture of HiBiT-tagged proteins without needing to overexpress the protein of interest.
The Anti-HiBiT Magne® Beads provide you with a fast and convenient procedure for enriching HiBiT-tagged proteins and associated complexes from mammalian, yeast and bacterial cells using IP and Co-IP methods. Using the Anti-HiBiT Magne® Beads opens more possibilities for your protein biology and mass spec-based proteomic studies.
The DrkBiT Peptide can be used to specifically and gently elute HiBiT-tagged proteins from the Anti-HiBiT Magne® Beads.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Anti-HiBiT Magne® Beads |
CS3278A07 | 1 × 1ml |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to (1) use in further material manufacture; and (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Anti-HiBiT Magne® Beads |
CS3278A09 | 1 × 5ml |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to (1) use in further material manufacture; and (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
DrkBiT Peptide |
CS3002A02 | 1 × 1.0ml |
SDS
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Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to (1) use in further material manufacture; and (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
Featured Resource
ASMS 2024 Poster Featuring IP-MS Data Generated with the Anti-HiBiT Magne® Beads
The combination of sensitive bioluminescent assays and the high-affinity antibody for quantitative immunoprecipitation makes HiBiT a uniquely powerful epitope tag.
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