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Measure Potency and Stability of Biologics that Bind and Block Canine PD-1/PD-L1 Interactions

  • No primary cell culture required: supplied in convenient thaw-and-use format
  • Consistency: standardized reagents improve results
  • Designed specifically for canine immunotherapeutic applications

Canine PD-1/PD-L1 Blockade Bioassay

  Catalog No. Product Name   Size Price Qty  
CS348807 Early Access Canine PD-1/PD-L1 Blockade Bioassay View Specifications 1 each     Please Enquire
CS348809 Early Access Canine PD-1/PD-L1 Blockade Bioassay 5X View Specifications 1 each     Please Enquire
CS348803 Early Access Canine PD-1/PD-L1 Blockade Bioassay, Propagation Model View Specifications 1 each     Please Enquire
CS348811 Early Access PD-1/PD-L1 Bioassay, Cell Bank View Specifications 1 each     Please Enquire

Early Access = This product is available under our Early Access program - Learn More

Catalog (FT) = This product is available under our Catalog (FT) program - Learn More

Overview
Protocols
Specifications
Resources
Related Products

Leading the Pack: Canine PD-1/PD-L1 Blockade Bioassay

Canine immune checkpoint therapeutics are a recent development in companion animal medicine, building on the success of immune-checkpoint inhibitor treatment in humans. Similar to their human counterpart, the canine immune system relies on checkpoint molecules on immune cells to control an immune response, but this mechanism has also been exploited by canine tumors to avoid detection. Emerging studies in veterinary oncology are focused on discovering and developing canine-targeted antibodies for immune checkpoints, such as CTLA-4 and PD-1/PD-L1.

There are several challenges to developing canine-specific antibodies for immune checkpoints, such as low-efficacy and low-throughput screening measures due to lack of standardized reagents and protocols, leading to labor-intensive assays with high levels of variability.

The Canine PD-1/PD-L1 Blockade Bioassay is a two-cell-line system, a target cell that expresses canine PD-L1 and an effector reporter cell that expresses canine PD-1. The assay is a biologically relevant MoA-based and robust system that can be used to measure the potency and stability of biologics designed to block canine PD-1/PD-L1 interaction. This bioassay reduces data variability and provides a convenient luminescent readout.

Demonstrate Canine PD-1/PD-L1 Blockade

Illustration depicting Canine PD-1/PD-L1 Blockade Bioassay assay principle.

Principle of Canine PD-1/PD-L1 Blockade Bioassay. The Canine PD-1/PD-L1 Blockade Bioassay consists of two genetically engineered cell lines, the CHO-K1 target cells and the PD-1 reporter effector cell. When co-cultured, the PD-1/PD-L1 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L1 interaction is disrupted, TCR activation induces luminescence that can be detected by addition of Bio-Glo™ Reagent and quantitation with a luminometer.


Assay Workflow: Standardized Reagents to Improve Your Results

Illustration depicting Canine PD-1/PD-L1 Blockade Bioassay assay workflow.
  • Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
  • Kit includes all of the required reagents in standardized formats.
  • Easily amenable to high-throughput workflows.

Assay Performance Data

Line graph showing results of Canine PD-1 Effector Cells co-cultured with Canine PD-L1 aAPC/CHO-K1 Cells in the presence of a titration of Anti-Canine PD-L1 Blocking Antibody.

Canine PD-1 Effector Cells were co-cultured with Canine PD-L1 aAPC/CHO-K1 Cells in the presence of a titration of Anti-Canine PD-L1 Blocking Antibody. After 6 hours, Bio-Glo™ Reagent was added and plates were read on a GloMax® Discover Luminometer.

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Protocols

No protocols available

Specifications

Catalog Number:

What's in the box?

Item Part # Size

Canine PD-1 Effector Cells

CS348804 1 × 0.5ml

Canine PD-L1 aAPC/CHO-K1 Cells

CS348805 1 × 0.5ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 1 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 1 × 1 vial

Fetal Bovine Serum

J121A 1 × 4ml

Ham's F-12 Medium

J123A 1 × 25ml

Certificate of Analysis

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Patents and Disclaimers

Promega Cell Line Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage and Transfer Restrictions:
    • 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
    • 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
  • 3. Propagation and Storage:
    • 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
  • 4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
    • 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
    • 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
      • 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
  • 5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
  • 6. For uses of HaloTag® Technology in this material, recipient must either:
    • 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 7. Disclaimer and Governing Law:
    • 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

What's in the box?

Item Part # Size

Canine PD-1 Effector Cells

CS348804 5 × 0.5ml

Canine PD-L1 aAPC/CHO-K1 Cells

CS348805 5 × 0.5ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 5 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 5 × 1 vial

Fetal Bovine Serum

J121A 5 × 4ml

Ham's F-12 Medium

J123A 5 × 25ml

Certificate of Analysis

Search by lot number

Patents and Disclaimers

Promega Cell Line Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage and Transfer Restrictions:
    • 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
    • 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
  • 3. Propagation and Storage:
    • 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
  • 4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
    • 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
    • 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
      • 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
  • 5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
  • 6. For uses of HaloTag® Technology in this material, recipient must either:
    • 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 7. Disclaimer and Governing Law:
    • 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

What's in the box?

Item Part # Size

Canine PD-1 Effector Cells (CPM)

CS348801 2 × 1ml

Canine PD-L1 aAPC/CHO-K1 Cells (CPM)

CS348802 2 × 1ml

Certificate of Analysis

Search by lot number

Storage Conditions

XX

Patents and Disclaimers

Promega Cell Line Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage and Transfer Restrictions:
    • 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
    • 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
  • 3. Propagation and Storage:
    • 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
  • 4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
    • 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
    • 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
      • 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
  • 5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
  • 6. For uses of HaloTag® Technology in this material, recipient must either:
    • 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 7. Disclaimer and Governing Law:
    • 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Certificate of Analysis

Search by lot number

Storage Conditions

XX

Patents and Disclaimers

Promega Cell Line Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage and Transfer Restrictions:
    • 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
    • 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
  • 3. Propagation and Storage:
    • 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
  • 4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
    • 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
    • 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
      • 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
  • 5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
  • 6. For uses of HaloTag® Technology in this material, recipient must either:
    • 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 7. Disclaimer and Governing Law:
    • 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Resources

Webinar: Precision in Veterinary Nucleic Acid Purification: Optimizing Complex Samples​

Join us for the first installment of our Veterinary and Animal Health webinar series, where our veterinary scientist Dr. Patrícia Eri Ishii, DVM, Ph.D., will demonstrate the power of advanced sample purification techniques. The webinar will explore how the Promega Maxwell Instrument simplifies the nucleic acid extraction and purification process across various complex samples in animal health diagnostics. Attendees will gain insights into using Promega chemistries to support diverse sample types and enhance the accuracy of diagnostic and research workflows.

Watch Webinar