Bio-Glo-NB™ Lytic Luciferase Assay Systems
Bright, Long-Lasting Luciferase Reagent for ADCP-MoA Target Cell Killing Assays
- Detection reagent designed for HiBiT target cell killing bioassays using primary macrophage
- Simple add-mix-read protocol for rapid assay results
- Quality controlled and tested for optimal reliability
Lytic Luciferase Assay Systems
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JB1101 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10ml | List Price: $ 240.00 Your Price: Log In | Add to Cart | ||
| JB1102 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 100ml | List Price: $ 1,410.00 Your Price: Log In | Add to Cart | ||
| JB1103 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10 × 100ml | List Price: $ 8,860.00 Your Price: Log In | Add to Cart |
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Quantify Targeted Cell Killing with Confidence
The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ Assay Reagents contain a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ Luciferase Assay Systems are functionally tested for performance in the Target Cell Killing Bioassays and intended for use with those products.
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Bio-Glo-NB™ Lytic Luciferase System: Assay Principle
The Bio-Glo-NB™ Lytic Luciferase Assay Systems are functionally tested for performance using ADCP-qualified macrophage in the Target Cell Killing Bioassays and intended for use with those products. When HiBiT expressing target cells are paired with primary macrophage and a biologic molecule, such as Rituximab, the target cells undergo antibody-dependent cellular phagocytosis (ADCP), which leads to degradation of HiBiT and decreased luciferase activity when LgBiT and the substrate are added.
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Simple Add-Mix-Read Protocol
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml | 20μM |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110A | 1 × 0.2ml | |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111A | 1 × 10ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml | 20μM |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110B | 2 × 1ml | |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 10 × 1ml | 20μM |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110C | 5 × 4ml | |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 10 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Featured Resource
The small size and bright luminescence of NanoLuc® luciferase bring exquisite sensitivity to many applications. From NanoBRET® technology, a BRET-based method to study protein:protein and protein:small molecule interactions, to NanoLuc® Binary Technology (NanoBiT), NanoLuc is the cornerstone for studying genetic responses and protein dynamics in cells at physiologically relevant levels. The flexibility of NanoLuc® technologies provides you with the bioluminescent building blocks to understand biological interactions or perform compound screening in ways that have never been possible before.
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