Bio-Glo-NB™ TCK Luciferase Assay System
Bright, Long-Lasting Luciferase Reagent for Target Cell Killing Assays
- Detection reagent designed for HiBiT target cell killing bioassays
- Simple add-mix-read protocol for rapid bioassay results
- Quality controlled and tested for optimal reliability
Catalog Number:
Size
$ 240.00
Catalog Number: JB1001
$ 1,410.00
Catalog Number: JB1002
$ 8,860.00
Catalog Number: JB1003
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Quantify Targeted Cell Killing with Confidence
The Bio-Glo-NB™ TCK Luciferase Assay System provides a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ TCK Assay Reagent contains a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ TCK Luciferase Assay System is functionally tested for performance in the Target Cell Killing Bioassays and is intended for use with those products.
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Simple Add-Mix-Read Protocol
Bio-Glo-NB™ TCK Results in a Brighter, Longer-Lasting Signal—Over 72 Hours
Luminescent signal is stable in assay medium for greater than 72 hours. Bio-Glo-NB™ TCK Reagent is optimized for batch and continuous-process handling; the extended bright light output offers high sensitivity, especially for extended incubations. HiBiT target cells were incubated with the respective CAR-T cells at the indicated time points before Bio-Glo-NB™ TCK Reagent was added. Panel A. Raji (HiBiT) cells. Panel B. H929 (HiBiT) cells.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Assay Substrate |
JB100A | 1 × 0.2ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101A | 1 × 10ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml | 20μM |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Substrate |
JB100B | 2 × 1ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101B | 1 × 100ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml | 20μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Substrate |
JB100C | 5 × 4ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101B | 10 × 100ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 10 × 1ml | 20μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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