Bio-Glo-NB™ TCK Luciferase Assay Systems
Bright, Long-Lasting Luciferase Reagent for Target Cell Killing Assays
- Detection reagent designed for HiBiT target cell killing bioassays
- Simple add-mix-read protocol for rapid bioassay results
- Quality controlled and tested for optimal reliability
TCK Luciferase Assay Systems
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JB1001 | Bio-Glo-NB™ TCK Luciferase Assay System | View Specifications | 10ml | List Price: $ 250.00 Your Price: Log In | Add to Cart | ||
| JB1002 | Bio-Glo-NB™ TCK Luciferase Assay System | View Specifications | 100ml | List Price: $ 1,480.00 Your Price: Log In | Add to Cart | ||
| JB1003 | Bio-Glo-NB™ TCK Luciferase Assay System | View Specifications | 10 × 100ml | List Price: $ 8,860.00 Your Price: Log In | Add to Cart |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Quantify Targeted Cell Killing with Confidence
The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ Assay Reagents contain a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ Luciferase Assay Systems are functionally tested for performance in the Target Cell Killing Bioassays and intended for use with those products.
Created with BioRender.com
Simple Add-Mix-Read Protocol
Bio-Glo-NB™ TCK Results in a Brighter, Longer-Lasting Signal—Over 72 Hours
Luminescent signal is stable in assay medium for greater than 72 hours. Bio-Glo-NB™ TCK Reagent is optimized for batch and continuous-process handling; the extended bright light output offers high sensitivity, especially for extended incubations. HiBiT target cells were incubated with the respective CAR-T cells at the indicated time points before Bio-Glo-NB™ TCK Reagent was added. Panel A. Raji (HiBiT) cells. Panel B. H929 (HiBiT) cells.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Assay Substrate |
JB100A | 1 × 0.2ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101A | 1 × 10ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml | 20μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Substrate |
JB100B | 2 × 1ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101B | 1 × 100ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml | 20μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Bio-Glo-NB™ TCK Luciferase Substrate |
JB100C | 5 × 4ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101B | 10 × 100ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 10 × 1ml | 20μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Related Products
Similar Products
CellTiter-Glo® Luminescent Cell Viability Assay
Determine the number of viable cells in culture based on quantitation of ATP present.
G7570, G7571, G7572, G7573, MG1010
T Cell Activation Bioassay (TCRαβ-KO)
Measure the potency of transgenic TCR constructs to activate T cells without the constraints of endogenous TCR expression.
GA1172, GA1162, GA1182, GA1210, GA1220, GA1230
FcγRI ADCP Bioassay
Measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRI.
GA1341, GA1345, GA1332, GA1330
Caspase-Glo® 9 Assay Systems
Measure caspase-9 activity with this homogeneous, luminescent assay.
G8210, G8211, G8212
Frequently Used With
H929 (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell death.
JA1511, JA1515, M2412, GA6080, CS3055A28, CS3055A30, CS3055B08, CS3055B09
Ramos (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell killing.
JA1411, JA1415, M2472, GA6070, CS3055A20, CS3055A22, CS3055B02, CS3055B05
Raji (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell killing.
JA1211, JA1215, M2452, GA6050, CS3055A14, CS3055A17, CS3055B16, CS3055B17, JA1251, JA1255, JA1261, JA1265, JA1271, JA1275
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
