QuantiFluor® ONE dsDNA System

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, "add-and-read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods but much more sensitive for low-concentration samples.

QuantiFluor Nanodrop Spectrophotometer DNA Extraction

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples and better or equal sensitivity performance when compared to Qubit® dsDNA HS. QuantiFluor® ONE Dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).

DNA Extraction & Purification with Better Sensitivity

	Sample*	Assay
QuantiFluor® ONE dsDNA System	0.2–400ng/µl	0.2–400ng
Qubit® dsDNA HS	0.2–100ng/µl	0.2–100ng
Qubit® dsDNA BR	2–1,000ng/µl	2–1,000ng

*Based on 1µl sample input per assay.

DNA Quantitation Assay Sensitivity and Linearity

The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA and is easy to set up on microplate or single-tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

Quantus™ Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

Discover Nanodrop Spectrophotometer DNA Extraction

GloMax® Discover

Explorer DNA Purification & Quantification Kit

GloMax® Explorer

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wieczorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Protocols

Complete Protocol

QuantiFluor® ONE dsDNA SystemPDF (1672 KB) – English

Quick Protocols

QuantiFluor ONE dsDNA System Quick Protocol FB200PDF (823 KB) – English

What's in the box?

Item	Part #	Size	Available Separately
QuantiFluor® ONE dsDNA Dye	E489A	1 × 20ml	View Product
QuantiFluor® ONE Lambda DNA	E490B	1 × 80μg
1X TE Buffer (pH 7.5)	E497B	1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.